FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_D07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_D07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951384
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA18490.19434844395112805No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC17920.188357172287951No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA16540.17385198826131193No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG15260.1603979045264583No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT15100.1587161440596016No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT14960.157244603651102No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC14250.14978179157942534No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT12750.13401528720264372No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA11570.12161230375957552No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC11170.11740790259243375No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC11000.11562103209639851No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA10520.1105757506958284No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG100.007030266145.41644145
AGCGAAA1600.063.4590231
GCGAAAG2050.049.5055542
ACCGTCA450.00965212148.316688
CGAAAGC2350.043.172073
TTCGGAC907.488084E-540.2723732
CCATCGG2151.2732926E-1137.0802469
TGTAACG800.002008093536.35411145
GACGATG1051.8226657E-434.622963145
GTAGAAA6450.033.732371
GTCTGAC5350.032.5192
GTTCGGA1153.1556984E-431.5324341
ATGAACG1153.1693568E-431.5092226
GCGATGT950.00473515330.5157998
CAATAGG7500.029.9768331
ACCATCG2701.72804E-1029.5268618
CACATCG1504.313275E-529.08329145
TGTCTCG1255.0678547E-429.08329145
GCTATTG6000.028.9900118
CTATTGA6150.028.2829369
Produced by FastQC (version 0.11.5)