FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588711
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA24720.4199004265250692No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC15310.26005968972891624No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG13260.22523785015058323No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG9150.1554243083618278No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG8760.14879966571034003No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG8720.14812021518198232No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA8090.1374188693603483No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA7880.13385175408647026No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC7750.13164353986930769No Hit
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT7630.12960518828423453No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA7350.12484903458573052No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC7300.12399972142528336No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT7270.1234901335290151No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT7090.12043260615140536No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG6980.11856411719842165No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT6920.11754494140588506No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC6350.1078627713767876No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT5910.10038881556485271No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT5900.10021895293276328No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG353.3840333E-583.11256145
AGCGAAA1850.074.46681
CGAAAGC2000.068.852523
GCGAAAG2600.055.7652442
CATTGCG400.005976430654.542614145
CCATCGG4300.052.2415059
ACCATCG4550.049.3710948
GACAGTG7200.043.2771267
GGACAGT7200.042.270686
GTACTAG700.001055774341.432651
ATGAACG2950.036.8461156
GACCATC6000.035.0242777
GCTTATG3550.034.7158242
TGAACGT3200.033.967517
GAACGTC3300.032.9381948
TATACCC1102.442043E-432.938195
AGTGATC11950.030.9260678
GGACCAT6800.030.9037766
GCAGGAC11300.030.7864073
GTGATCA12050.030.068069
Produced by FastQC (version 0.11.5)