FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060817
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80700.7607344150781897No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG64830.611132740142739No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA60580.571069279621273No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA57430.5413751853524218No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG45220.4262752199483983No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG43110.40638489013656454No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT39970.37678506283364616No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT39170.36924370555901725No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC36190.3411521497110246No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT35340.3331394576067314No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC34850.3285203762760212No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT34450.3247496976387068No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT32220.3037281642356787No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG31740.2992033498709014No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC31080.29298173011933254No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT30630.28873971665235376No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG29790.2808212915139935No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC26540.25018452758581355No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG25520.24056929706066174No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA24440.23038846473991273No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT24400.23001139687618127No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG24400.23001139687618127No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG23760.22397831105647817No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC22540.21247774121266907No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC22520.2122892072808034No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG21950.2069159902226303No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG21740.20493638393804023No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20590.19409568285576118No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG20350.1918332756733725No Hit
CAAGCCAAATTCATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG20030.18881673276352093No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC18840.17759896381751047No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA18690.17618495932851755No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA18160.1711888101340759No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT17380.16383598679131273No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA17360.163647452859447No Hit
TTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATG17020.16044237601772973No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC16690.15733156614194532No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG16160.15233541694750366No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA16130.1520526160497051No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA16090.15167554818597365No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT15700.14799913651459207No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA15580.14686793292339773No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG15090.14224885159268752No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA14980.14121191496742605No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG14940.1408348471036946No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT14930.14074058013776175No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA14520.13687563453451446No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT14410.13583869790925296No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC14370.1354616300455215No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA14130.13319922286313285No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG14080.13272788803346855No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC14000.13197375230600564No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC13990.1318794853400728No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA13910.1311253496126099No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACACAGAGACTGGTGC13860.1306540147829456No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13830.130371213885147No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG13820.13027694691921415No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13690.12905147636208697No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG13680.1289572093961541No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13660.12876867546428836No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT13650.1286744084983555No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC13410.12641200131596686No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC12990.12245278874678668No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG12780.12047318246219658No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC12660.11934197887100224No Hit
TCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACT12650.1192477119050694No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12600.1187763770754051No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA12450.11736237258641218No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT12370.11660823685894928No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT12370.11660823685894928No Hit
GTAAACATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGAT12260.11557130023368781No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC12230.11528849933588922No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA12120.11425156271062775No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11850.11170635463044051No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA11750.11076368497111189No Hit
GTTCATTGATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTC11720.1104808840733133No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA11580.10916114655025325No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA11400.10746434116346176No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11390.10737007419752889No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11350.10699300633379744No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11270.10623887060633454No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11220.10576753577667024No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG11120.10482486611734163No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT10910.10284525983275156No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA10840.10218539107122152No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10780.10161978927562434No Hit
GAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTAC10770.10152552230969149No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT10610.10001725085476572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA151.215536E-4145.43196145
GACGTGG100.0070281397145.43195145
TGACGCG202.0993539E-6145.43195145
CGAGTTC100.007103982144.910783
TCGGACA100.0071049836144.903954
AGATCGG351.8644641E-9124.65595145
AGTAGAA8750.0116.021641
TCTTGCG404.7166395E-9109.07396145
ATAGACG203.8206417E-4109.07396145
TTTTTCG203.8206417E-4109.07396145
GTTGGCG203.8206417E-4109.07396145
CCGCACG800.0109.07396145
AGCGAAA1650.0105.474211
GCAGGTA4350.099.999772
ATCATAG301.5760494E-596.95464145
CAGGTAC4650.093.490833
TACATCG406.010341E-790.894966145
ATACCGG259.379953E-487.016231
TACCGGA259.3869894E-486.99982
CGCTGCG353.3870274E-583.103966145
Produced by FastQC (version 0.11.5)