FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027992
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2500.063.7892531
TGACGCG450.00952564348.478542145
CGAAAGC3650.043.6785163
CCGTATA1051.8213553E-434.627533145
CAATTCG2008.974894E-932.616339
GTATATA9450.029.1485271
TAATACT7550.025.9139584
CGCACTA2259.129071E-725.7709268
GCGAAAG6500.025.647032
TATATAC9950.025.4907723
ACGCACT2601.1482916E-725.088267
GTATAGG2901.19289325E-824.9957891
ATATACT6600.024.1543664
ATAATAC6450.022.4702723
GAGTATA2603.2089956E-622.3039341
TATATAT9200.022.0593532
CCGACGC1650.00255599422.035702145
TATAATA5400.021.475772
ACTAATA4751.2732926E-1121.3627412
TATACTT8500.021.3137135
Produced by FastQC (version 0.11.5)