FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577702
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCG100.0070235645145.45792145
AGCGAAA2700.096.711531
CGAAAGC3250.080.296283
TAACGCG350.003567748562.134035
CGTATTG400.00605507354.3625689
ATGGTCG553.1681493E-452.893784145
TCGCCGG858.726274E-751.33809145
AACGCGA450.00964250148.326476
GCGAAAG5750.045.3848532
TTAACGC657.336005E-444.6129074
TCTATAC1905.4260454E-934.3372273
CTATTAA2603.6379788E-1233.477071
CATATAT3100.032.7571331
TAGACGA1352.1427266E-532.217645
ATCCGGA950.004728946430.5219786
AGCTTGC2151.8102583E-830.3445245
TAATACT1703.0101128E-629.8512844
GAATACG1703.0121464E-629.84876
GTATATA2701.70985E-1029.5507471
GAGCGAA1000.006058606329.0134621
Produced by FastQC (version 0.11.5)