FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37224
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAG7311.9637868042123359No Hit
TCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAG3961.0638297872340425No Hit
GTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTG3020.8113045347087902No Hit
GTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATTCCCTTCCCCC2950.7924994627122286No Hit
AAGCAGGGGTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAG2150.5775843541800989No Hit
GTCCAGGACCTTTTCAAGGCTGTCTCCATGGCAACGACAATCTTGTGAAC2140.5748979153234473No Hit
GGTACAGACGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGC2070.5560928433268859No Hit
GTACATGGACACGGCATTCTGGGAAAAGTCAAAGAGAACACGGAAAACTT2040.548033526756931No Hit
GTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCC2010.5399742101869762No Hit
GTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAGACAG1840.49430474962389853No Hit
ATGCTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTCAAC1740.4674403610573823No Hit
GTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACTTT1670.44863528906082095No Hit
ATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTG1670.44863528906082095No Hit
GGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAGACAGATTCTCAGTTC1600.4298302170642596No Hit
ATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCC1600.4298302170642596No Hit
AGCAGGTACAGACGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGA1550.4163980227810015No Hit
TTTCAAGGCTGTCTCCATGGCAACGACAATCTTGTGAACTGAGAATCTGT1540.41371158392434987No Hit
GGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACT1500.40296582849774343No Hit
TCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAG1500.40296582849774343No Hit
CCTGTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCATGG1430.38416075650118203No Hit
GAGTAGGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAGACAGATTCTC1390.37341500107457554No Hit
TATGTATCCAGTGAGATGCGCTCCGGCCCCATCCTGATTCTCCTGAAGGG1370.3680421233612723No Hit
GTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGT1330.3572963679346658No Hit
AAGCAGGAGAGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAA1300.34923705136471095No Hit
CCCCTAGGCCCAATCAGACAGATTCTCAGTTCACAAGATTGTCGTTGCCA1270.3411777347947561No Hit
GTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAG1260.33849129593810445No Hit
ATGCATATCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGT1240.3331184182248012No Hit
CCCTTCCCCCTTTCCACAGACACCTGTGGATTGTTGTCTCTGTCCAGGAC1230.3304319793681496No Hit
GCATTGAACATTGATGACCTTCAACCTCATATTAGTGAGGTACAACATCG1210.3250591016548463No Hit
GCGTCATGCTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCT1190.3196862239415431No Hit
GTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTG1180.31699978508489146No Hit
GGTGAATGCATATCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAG1120.3008811519449818No Hit
GTGTTAAACTTTTGTTGTTTTCGTTGGGTTGCCGTGGAGGAGGGAAGTCA1110.2981947130883301No Hit
GCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATT1100.29550827423167847No Hit
TGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAGGC1090.2928218353750268No Hit
CTATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTCAACCTC1060.284762518805072No Hit
AATAAGTAGTGTGTGAAAGAAAAACGAGAAAGAGAGAGAGAGAGAGAGAG1060.284762518805072No Hit
GGGTATGACTGAGTGACTAATTGGCTTGTCTGCCTGATCTGGCGCATACT1030.27670320223511713No Hit
CTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGT970.2605845690952074No Hit
GAAATAAGTAGTGTGTGAAAGAAAAACGAGAAAGAGAGAGAGAGAGAGAG970.2605845690952074No Hit
GTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGA930.24983881366860092No Hit
GATCATATGTATCCAGTGAGATGCGCTCCGGCCCCATCCTGATTCTCCTG930.24983881366860092No Hit
GGATGGGGGTCGTGGGGTGGTGAATGCATATCTGTAATATGTACGTGTGT930.24983881366860092No Hit
TATCTGTAATATGTACGTGTGTATGTATGCATGTAGAAAGTTTGTGTATG920.24715237481194927No Hit
CACCTGGACGACATCCTGCCTCTCTTGTTGCAGGCTAGCACAGACAGCCA880.2364066193853428No Hit
ATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTG840.2256608639587363No Hit
GAACATTGATGACCTTCAACCTCATATTAGTGAGGTACAACATCGCAGTA840.2256608639587363No Hit
CAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGTAAGCGATCTTCAC830.22297442510208465No Hit
AAGCAGGACAGACACAGGGACAGACGTGATCATATGTATCCAGTGAGATG820.22028798624543305No Hit
CCCCTGGCCCCAGCGCGTCCGACACACCCACAGAAAACAATAAACTTTAG810.21760154738878143No Hit
TTTGTTGACTGCATTGAACATTGATGACCTTCAACCTCATATTAGTGAGG800.2149151085321298No Hit
GCAGGGGCCAGTCCGCCCCTGGCCCCAGCGCGTCCGACACACCCACAGAA750.20148291424887171No Hit
CAGGAGAGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGC750.20148291424887171No Hit
GTACAGACGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGCA750.20148291424887171No Hit
AAGCAGGGGAGGGTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATG710.1907371588222652No Hit
ATGTATGCATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCCTT710.1907371588222652No Hit
ATCTTCACCTGAGCAGGGGTTCACGTTGCTCGGTAGGTCTAACCTACCTT700.1880507199656136No Hit
GGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACTTTTGTTGTTTTCGT690.18536428110896197No Hit
CTGTTGGGCTTGGCTGTGCTGCCGCCTGTTATTTATTGCAGAGCTGGCTG690.18536428110896197No Hit
AGCGAAAGCAGGGGTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAAC680.18267784225231035No Hit
GGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCC680.18267784225231035No Hit
GGGGAGGGTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGCAGC670.17999140339565872No Hit
GAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGACTCTGGCATTCCCTT670.17999140339565872No Hit
AAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGTTAAACTTTTGTTGTT660.1773049645390071No Hit
GGCCAGTCCGCCCCTGGCCCCAGCGCGTCCGACACACCCACAGAAAACAA660.1773049645390071No Hit
CAGGAAGGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTGT660.1773049645390071No Hit
AGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAAAACC650.17461852568235547No Hit
CACCTGTGGATTGTTGTCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCAT640.17193208682570385No Hit
CTGCAGTGAGACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTGTGG640.17193208682570385No Hit
GCCCCAGCGCGTCCGACACACCCACAGAAAACAATAAACTTTAGTAAGCC630.16924564796905223No Hit
CATTTGTTGACTGCATTGAACATTGATGACCTTCAACCTCATATTAGTGA630.16924564796905223No Hit
GACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGCATTGAACATT630.16924564796905223No Hit
GTCAAAGAGAACACGGAAAACTTTTAGTCAGGACTACTGCGATGTTGTAC620.1665592091124006No Hit
TCTTAAAGGCAAAACCCAGGCGGCAGTACATGGACACGGCATTCTGGGAA610.16387277025574898No Hit
GGATTGTTGCCTTCCCCTAGGCCCAATCAGACAGATTCTCAGTTCACAAG600.16118633139909735No Hit
TCGCTGCAGTGAGACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTG600.16118633139909735No Hit
CAGTGAGACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTGTGGATT600.16118633139909735No Hit
AGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTG590.15849989254244573No Hit
GGACAGACACAGGGACAGACGTGATCATATGTATCCAGTGAGATGCGCTC590.15849989254244573No Hit
CTTTAGTAAGCCGGAAAAACAACCAGCGGTAAGCGATCTTCACCTGAGCA590.15849989254244573No Hit
CCCTAGGCCCAATCAGACAGATTCTCAGTTCACAAGATTGTCGTTGCCAT580.15581345368579413No Hit
CCTTCAACCTCATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAA580.15581345368579413No Hit
GGTTAGATTGAGTGCAGGTGTAGTGGTAACTGGGTGGGAAAAGGTAGGTT580.15581345368579413No Hit
ATTTATTGCAGAGCTGGCTGGGAGCTCAGCATGGTGTCTGCAAACTGGGT580.15581345368579413No Hit
GTCCTGGACAGAGACAACAATCCACAGGTGTCTGTGGAAAGGGGGAAGGG580.15581345368579413No Hit
CACCCACAGAAAACAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGT570.15312701482914248No Hit
GGAGAGGTGAAGCTGTTCTGGACTGATCACCTGGCCCTTCTTAAAGGCAA570.15312701482914248No Hit
GATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACATGGACA560.1504405759724909No Hit
GAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGTGAGA560.1504405759724909No Hit
CTCCGGGACTTACGCAACCCTTCAGGAGAATCAGGATGGGGCCGGAGCGC560.1504405759724909No Hit
GTCGTGGGGTGGTGAATGCATATCTGTAATATGTACGTGTGTATGTATGC550.14775413711583923No Hit
GCAGGAAGGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGTG540.14506769825918764No Hit
GGCATTCCCTTCCCCCTTTCCACAGACACCTGTGGATTGTTGTCTCTGTC540.14506769825918764No Hit
GTCCGCCCCTGGCCCCAGCGCGTCCGACACACCCACAGAAAACAATAAAC540.14506769825918764No Hit
GGACTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACAT540.14506769825918764No Hit
CTCCATGGCAACGACAATCTTGTGAACTGAGAATCTGTCTGATTGGGCCT530.142381259402536No Hit
AGCACACACAGCCCGTGGGTATGACTGAGTGACTAATTGGCTTGTCTGCC530.142381259402536No Hit
CCCACAGAAAACAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGTAA520.1396948205458844No Hit
ATGTGACTCATTTGTTGACTGCATTGAACATTGATGACCTTCAACCTCAT520.1396948205458844No Hit
CAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTGACTTTTCCCAGAA520.1396948205458844No Hit
GTTTGTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAGGCCCAATCAG510.13700838168923274No Hit
GCAGGGGAGGGTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGC510.13700838168923274No Hit
CACAGGGACAGACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCCCC510.13700838168923274No Hit
CACACACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGTGA510.13700838168923274No Hit
GTTCACAAGATTGTCGTTGCCATGGAGACAGCCTTGAAAAGGTCCTGGAC510.13700838168923274No Hit
CTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGCAGT510.13700838168923274No Hit
CTTTTGTTGTTTTCGTTGGGTTGCCGTGGAGGAGGGAAGTCAAATTTGAA500.13432194283258114No Hit
CTGATCACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACATGGA500.13432194283258114No Hit
GCAGTACATGGACACGGCATTCTGGGAAAAGTCAAAGAGAACACGGAAAA500.13432194283258114No Hit
GACACAGGGACAGACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCC500.13432194283258114No Hit
CACCTGGCCCTTCTTAAAGGCAAAACCCAGGCGGCAGTACATGGACACGG490.1316355039759295No Hit
GTCCCGGAGTATGCGCCAGATCAGGCAGACAAGCCAATTAGTCACTCAGT490.1316355039759295No Hit
GGACAGACGTGATCATATGTATCCAGTGAGATGCGCTCCGGCCCCATCCT480.1289490651192779No Hit
GGCAAAGGCGTGGGATGGGGGTCGTGGGGTGGTGAATGCATATCTGTAAT480.1289490651192779No Hit
CGGACAAGCTGCGTCATGCTATGTGACTCATTTGTTGACTGCATTGAACA470.12626262626262627No Hit
CTTGTGAACTGAGAATCTGTCTGATTGGGCCTAGGGGAAGGCAACAATCC470.12626262626262627No Hit
CACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGTGACTAA460.12357618740597463No Hit
CAATCAGACAGATTCTCAGTTCACAAGATTGTCGTTGCCATGGAGACAGC460.12357618740597463No Hit
GCCGTGGAGGAGGGAAGTCAAATTTGAAAGTGACGGCAGCTGTGGGCTCT460.12357618740597463No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG460.12357618740597463No Hit
CTTAAAGGCAAAACCCAGGCGGCAGTACATGGACACGGCATTCTGGGAAA460.12357618740597463No Hit
TTGATGACCTTCAACCTCATATTAGTGAGGTACAACATCGCAGTAGTCCT450.12088974854932302No Hit
GTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGTGTTCTCTTTGAC450.12088974854932302No Hit
CACACACACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGT450.12088974854932302No Hit
GCGGTAAGCGATCTTCACCTGAGCAGGGGTTCACGTTGCTCGGTAGGTCT440.1182033096926714No Hit
GTTATTTATTGCAGAGCTGGCTGGGAGCTCAGCATGGTGTCTGCAAACTG440.1182033096926714No Hit
GTGTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGCAGCGTGTCCT440.1182033096926714No Hit
CATATTAGTGAGGTACAACATCGCAGTAGTCCTGACTAAAAGTTTTCCGT430.11551687083601977No Hit
GTCCTACTTTCCAGCAGGGAGATGCTGTTTTTCATGGCAGCGTGTCCTTC430.11551687083601977No Hit
AGTATGCACAGCCTGTTTCTCTCCCAACGAGAGCCCACAGCTGCCGTCAC420.11283043197936815No Hit
GTTCTTGGAACAGTTTGGCGCTGAACAGTTTTAAAACTGAAGATCGCTGC410.11014399312271653No Hit
ATCCACAGGTGTCTGTGGAAAGGGGGAAGGGAATGCCAGAGTCTCACTGC410.11014399312271653No Hit
TCTCTACCTCGGGAGGAGAGTCTGTGGGGAAAACGGCTTCCCCAAGGTGA410.11014399312271653No Hit
AAAGCAGGCAGCTGTTGGGCTTGGCTGTGCTGCCGCCTGTTATTTATTGC410.11014399312271653No Hit
ACAGAAAACAATAAACTTTAGTAAGCCGGAAAAACAACCAGCGGTAAGCG400.1074575542660649No Hit
CATGTAGAAAGTTTGTGTATGAGTAGGCCTGGATTGTTGCCTTCCCCTAG400.1074575542660649No Hit
GCCTTGAAAAGGTCCTGGACAGAGACAACAATCCACAGGTGTCTGTGGAA400.1074575542660649No Hit
CACACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGAGTGACT400.1074575542660649No Hit
AGCAGGAAGGGTATTGCAAGTTGGGGAGACCCACGCGGAAAATTGTAAGT390.10477111540941328No Hit
GCTGTTGGGCTTGGCTGTGCTGCCGCCTGTTATTTATTGCAGAGCTGGCT390.10477111540941328No Hit
GACTCTGGCATTCCCTTCCCCCTTTCCACAGACACCTGTGGATTGTTGTC390.10477111540941328No Hit
GCCCGTGGGTATGACTGAGTGACTAATTGGCTTGTCTGCCTGATCTGGCG390.10477111540941328No Hit
GTGCATACTGGTTAGATTGAGTGCAGGTGTAGTGGTAACTGGGTGGGAAA380.10208467655276166No Hit
CACACACACACACACACACGCCAGCACACACAGCCCGTGGGTATGACTGA380.10208467655276166No Hit
TCTCTGTCCAGGACCTTTTCAAGGCTGTCTCCATGGCAACGACAATCTTG380.10208467655276166No Hit
GGCGTGGGATGGGGGTCGTGGGGTGGTGAATGCATATCTGTAATATGTAC380.10208467655276166No Hit
CCACTACACCTGCACTCAATCTAACCAGTATGCACAGCCTGTTTCTCTCC380.10208467655276166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG100.0069667604145.67027145
ACCATAT100.0070801075144.88718
GGACCAT100.0070801075144.88716
CGTGGAC100.0070801075144.88713
AGCAGCG100.0070801075144.88717
GCAGCGG100.0070801075144.88718
AGCGAAA400.0144.88711
GCAGGTA301.08580934E-7120.739242
AGCAGGT301.08580934E-7120.739241
CAGGTAC301.08580934E-7120.739243
CTTGGCT501.8189894E-12115.9096769
GGCTTGG501.8189894E-12115.9096767
TGGGCTT458.185452E-11112.6899645
GTTGGGC458.185452E-11112.6899643
CTGTTGG458.185452E-11112.6899641
GGGGGGA404.5401976E-9109.2527145
GCTTGGC553.6379788E-12105.372448
TTGGGCT501.891749E-10101.420974
GGACTGA1650.083.419846
GTTCTGG1650.083.419841
Produced by FastQC (version 0.11.5)