FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705330
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT7150.10137098946592375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1950.055.7730831
GCCGTAG701.6651049E-551.94408145
GAACCGT604.9510534E-448.3195276
CGAAAGC2300.047.2657473
GCGAAAG2350.046.2633742
TGTCTCG1352.0970563E-532.320763145
GAACGTC1402.7509E-531.0625538
GCATTAA4950.029.2949491
ATGAACG1555.508866E-528.05656
ATTTACG1050.007720357727.6091983
TTTACGG1050.007720357727.6091984
ATAGTAC3005.875336E-1026.5738543
TGAACGT1658.4314815E-526.3561067
GTACACC6900.025.20843
AACGTCA1450.001228128124.9928599
TAGAGTG2951.43299985E-824.5657675
TACCATG2701.651988E-724.1597637
GTATTAT7300.023.8372611
TACACCC6400.023.780584
GTAGAAA5200.023.703561
Produced by FastQC (version 0.11.5)