FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_C02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_C02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569327
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6980.12260089544321628No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA5880.10327983742207905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG259.407389E-486.947025
ATGAACG1007.221388E-1057.9646846
CAATTCG2150.057.2957089
AGCGAAA1405.456968E-1251.799691
GCGAAAG1652.910383E-1143.9203572
AGTAGAA4300.042.1625371
CTTTCCG700.001039879441.56034145
CGAAAGC1807.2759576E-1140.256793
GTAGTAC907.5013806E-540.256793
GTTATAC2353.45608E-1133.9184843
CCATCGG1957.015842E-933.44419
GGATAGC1551.4705183E-632.7225
GTAGAAA5550.032.6435052
GTATTAA1952.597808E-729.7516141
CGGACAA200.0060797928.99660745-49
ACAATTC4250.028.984898
ACCATCG2105.0028393E-727.6046568
TGAACGT2207.5105345E-726.34997
GAACGTC2207.5105345E-726.34998
TTATACT6100.026.1316224
Produced by FastQC (version 0.11.5)