FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369777
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA10100.27313759373892915No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT7800.21093794367956906No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC7300.1974162806231864No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA7010.18957371605048448No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG6980.18876241626710152No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT5680.15360609232050668No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC5580.15090175970923017No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG5500.14873829362020893No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC5470.14792699383682598No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC5340.14441136144216649No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC5280.14278876187540057No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA5160.13954356274186874No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT4750.12845579903563498No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT4720.12764449925225205No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG4690.12683319946886906No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC4640.1254810331632308No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC4600.12439930011872022No Hit
ATATTCAACTGTCTCTCCTTTGACCGATGTTCGAGTTATGTTTTGTTTTC4590.12412886685759254No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG4470.12088366772406071No Hit
GGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAAC4420.11953150141842245No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT4340.11736803532940124No Hit
ACCCAGGACCTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTAT4320.11682716880714591No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT4260.11520456924038No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG4250.11493413597925235No Hit
GAAGTGGGATCATACGTTTATTTTGCACTTCATTTTTCCCACTAAATAAT4220.11412283619586941No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA4200.1135819696736141No Hit
ATGTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAAC4170.11277066989023114No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA4090.11060720380120992No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG4070.11006633727895462No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT4060.10979590401782698No Hit
ATTTTAAAAGGTGCATTAGATTTATCCCTACCCAGGACCTATTATTTAGT4030.108984604234444No Hit
CTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTATGATCCCACT4020.10871417097331636No Hit
GTATAATACAGTGATTAGACAAGTCTATAAGTTATGCTGTGCTCAGGAAA4000.10817330445106103No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG3900.10546897183978453No Hit
CTTATAGACTTGTCTAATCACTGTATTATACATCTGAAACTAATGTAACA3880.1049281053175292No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG3810.10303507248963564No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG3780.10222377270625267No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT3750.10141247292286973No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG100.007013587145.52002145
AGAATCG100.007013587145.52002145
AGCGAAA606.7948804E-660.4200441
TAGCGTT400.005962813754.570007145
CAATGCG400.006056257554.355989
GTAGAAA2300.053.589961
CCCCGAG1405.456968E-1251.76769
CTAATAT1152.4792826E-950.4307863
ACTAGTA604.949543E-448.3164258
AGTAGAA1900.045.7920381
GGTCTGA1751.8189894E-1245.5739821
GCGAAAG855.333173E-542.6494451
TCTAATA1401.4097168E-841.4308932
CCATCGG1755.2750693E-1141.414089
CGAAAGC907.470809E-540.280032
ACCATCG1859.640644E-1139.1754848
GTCTGAC1701.8226274E-938.3845022
ATGAACG951.02975304E-438.144556
TAGAAAC3600.036.252032
TAGCATG1209.570482E-636.237327
Produced by FastQC (version 0.11.5)