FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679522
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACATCTGAGGGAAAAAACTGCAAAAATGTCCCCAAATCTGTGGAAA24030.35363093468644136No Hit
GAGTATGAGAAGTATATGAGAAAATGATGCAGAATATACATCTGAGGGAA15170.22324516351199813No Hit
GTATATGAGAAAATGATGCAGAATATACATCTGAGGGAAAAAACTGCAAA14920.2195661067632836No Hit
GAATATACATCTGAGGGAAAAAACTGCAAAAATGTCCCCAAATCTGTGGA13080.1924882490927446No Hit
TCTCTAAGCAGTTACTTCAGACGACTTTCTCTTTTCCTCTTAGAACTTTA12890.18969216596372154No Hit
GTATTTATGTTGTTAGGTCAGCCAAGGATTTGAATGTAAATCTTTGTACC12450.18321702608598397No Hit
CTTTAGTATTTCTTGTACTTCAGATCTCTAAGCAGTTACTTCAGACGACT12080.17777202209788645No Hit
GTATTAGAAAATGTACGAGATGTGCTGTAGAAGTTTTGGTTTCTCTCTAT10940.16099552332374817No Hit
GAATATATGCAAATCCAATGATCTCAGTAATCTCTAAGCAGGAAAAATAC10470.15407889663616484No Hit
TTCTAGTACTGAACAAATCTTGACTTTCTTTGGAAAAGCTGAACAAAAGA10110.1487810549180159No Hit
CTATAGAGTTTTGTTTTTTGCTCTGGTAAGTAGTTACATTATTGGTCAAT9800.1442190245496099No Hit
CTTCTATAGTGCTTATTGTGTGCTAGGCACTGTTCTAAGCAGTTGTACAC9200.13538928835269498No Hit
TTCTTGTACTTCAGATCTCTAAGCAGTTACTTCAGACGACTTTCTCTTTT9000.13244604295372334No Hit
GCCTAGGGAGGGGGGACAGCACCGGTTACCTGGCAAGCAGAGGTGGCAGC8850.13023860890449462No Hit
TCCTTATAGCTACTGTAATTTTTAAACTTTCAAAGGATATTACCATTCGT8510.12523509172624286No Hit
GTATATAGTGAAGGACCAGGCTCTGCCCAAGAAACTTTGGTCAAGTTTTC8160.12008441227804251No Hit
ATTATAAACTATGGACAAAATTTTTTAAAAACTATTTGAAGGCAAGGAAG7950.1169940046091223No Hit
CCTTATAGCTACTGTAATTTTTAAACTTTCAAAGGATATTACCATTCGTC7910.11640535552932796No Hit
TCCTAGAGCTGCGTGGGCGCCCCTCCAGGCCCTCTAGCACCTTCCCTCCT7860.11566954417958507No Hit
GCATAAAGAGGGACACCAAAGAGAACATGAAAAGAGGCACAATATCACTT7810.11493373282984215No Hit
ACCCTGCACACAACCCACAGAGTCTTTGGCCTGGAGGCCTCTTGAGAGGG7760.11419792148009925No Hit
GGATATTACCATTCGTCTTTCAATGTTAAAGTAGCAAACACTTCTATAGT7680.11302062332051059No Hit
CTCTAGCACCTTCCCTCCTGCACTTCCCTCTAGATGCTGCGGAGGCAGCC7630.11228481197076767No Hit
GTCTTTGGCCTGGCGTTGTGGATCTAGTGCTCATTCAGTCCCCGCAGCAA7550.11110751381117905No Hit
GTTCTAAGCAGTTGTACACATCTTAACCCACTTAATCCTCATAGCCACCT7540.11096035154123045No Hit
GGTAAGTAGTTACATTATTGGTCAATTATCTGGAGGCTTGGTTTTATGCT7320.10772278160236165No Hit
CTGTTAGAACAGGTTTGTAGAAAGCCCAAATTATTTACCAAGCATCCCTG7150.10522102301323577No Hit
ATTTTATACACTAGGAAAAAAAAAATGAAGACCTTCAAGTCAGAAAAACT7070.10404372485364713No Hit
ATATAAAGGACTCCTGTGGGTCTGTGGCAAGATAATAGACAACCCAACTC6950.10227777761426414No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG100.0070222756145.46872145
TACCGTC203.8669145E-4108.740122
AGCGAAA1800.092.664571
TAGATCG406.555968E-572.73436145
ACCGTCC300.001936737372.4880753
CCGACGG553.9831557E-666.12215145
CTAGTAC2600.064.124073
TAACGGA350.003570417662.123499
GAGTATG4050.060.8810731
CGCACAA501.977973E-458.187485145
AGTATGA4300.057.3203742
AACTATG2250.054.756677
CTTTCCG400.005973291654.550774145
TCGTCGT400.006057518554.3580556
TAGTACT3100.053.7775154
CGAAAGC3150.052.92783
TATAAAC3650.051.635343
GTATGAG4850.050.816383
GTACTGA4000.048.922256
GAGCGAA450.00962726548.3467331
Produced by FastQC (version 0.11.5)