FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663133
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT7170.10812310652614182No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6640.10013074300328895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG451.17403324E-464.659615145
CGATACG450.00965470948.311789
CGAAAGC2500.046.375812
TTAGCCG657.215322E-444.764347145
CCTCGCG657.215322E-444.764347145
GCGAAAG2800.041.431981
GGTCGTT1752.4392648E-937.2662777
CAATTCG2850.033.055439
AGGTCGT2008.985808E-932.6079946
TATGCGT1601.8835035E-631.7022156
TACTATA1703.0219508E-629.837382
CTATAAG2451.9681465E-929.5942691
ATGCGTC1753.7874343E-628.9848827
TTATACT6350.028.5241224
ATATACT4850.028.3829734
TATACTG5950.028.0084865
TATATAC7550.027.8289663
GTAGGAC1855.842714E-627.4139983
GTCGTTT2405.2927135E-827.1733288
ATATGCG1907.183902E-626.6945885
Produced by FastQC (version 0.11.5)