FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630910
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG57680.35366758435474677No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40120.24599763322316986No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC38530.23624847477788474No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA37330.22889061934748084No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA36300.22257512676971755No Hit
GGGTAGTAGAGGGTAGATGCAGTCCAGCTAGCTGTCTGCCACTGGGCTGG35740.21914146090219572No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT33360.20454838096522798No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT32430.19884604300666497No Hit
CTTTCAGGTAGGGTAGTAGAGGGTAGATGCAGTCCAGCTAGCTGTCTGCC31190.19124292572858098No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT30240.1854179568461779No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA30050.1842529630696973No Hit
GCTTTAAGGATTCTTTGGTGTTTGTTGAGACTTATGTTGTGGTCTGATGT29560.1812485054356157No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG28440.17438117370057207No Hit
ACCTACAAGCGATATACAAGAAAGAGATCTAAACACAAAGACATAGGAGA27520.1687401512039291No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT27130.16634884818904783No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG26750.1640188606360866No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG25060.15365654757160113No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT24730.15163313732824005No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC24600.15083603632327963No Hit
ATATACAAGAAAGAGATCTAAACACAAAGACATAGGAGAATAAAAGTAAA24320.14911920338951873No Hit
GACATAGGAGAATAAAAGTAAATGGAGGAGGAAACATAGTCGGCCAACCT23170.142067925268715No Hit
AAATAAAGCCTTACCTACAAGCGATATACAAGAAAGAGATCTAAACACAA22920.1405350387207142No Hit
TTTCTATCCCATCATTTTGCTGGCTTTGTCTTTGCTTGTTTTTTTCTTTT22460.1377145274723927No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC22100.13550717084327155No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG21730.13323849875223034No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG21650.13274797505687008No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT21320.13072456481350903No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC21050.12906904734166813No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20990.12870115457014794No Hit
CCCTTACTCATTCTCCATTGCTTTAAGGATTCTTTGGTGTTTGTTGAGAC20620.12643248247910674No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20160.12361197123078527No Hit
TTATTAAGCTTGATCCATGAATATATTTCAAACACTGCTCCTAAGAACTT19900.12201776922086442No Hit
GGGTAGATGCAGTCCAGCTAGCTGTCTGCCACTGGGCTGGTGGGAGACTC19770.12122066821590402No Hit
GTAGTAGAGGGTAGATGCAGTCCAGCTAGCTGTCTGCCACTGGGCTGGTG19360.11870673427718267No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC19290.11827752604374245No Hit
GTTCTTAGGAGCAGTGTTTGAAATATATTCATGGATCAAGCTTAATAACG19090.1170512168053418No Hit
GATATACGCATGTGCAAGTCTTAGCTTGTAAAATAACACCCCACACACTC18810.11533438387158089No Hit
ATACAAGAAAGAGATCTAAACACAAAGACATAGGAGAATAAAAGTAAATG18790.11521175294774083No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA18740.11490517563814066No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC18700.11465991379046053No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT18600.11404675917126021No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT18580.11392412824742017No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG18430.11300439631861967No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC18370.11263650354709946No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA18350.11251387262325942No Hit
GGGTGGGGTCTCAAAGCTCTTGGGCCCAGTGGACCACAGCACCACCAGAG18330.11239124169941934No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA18270.11202334892789914No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA17970.11018388507029818No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT17750.10883494490805745No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA17440.10693416558853645No Hit
CCTCTGGGTCACTTTCAGGTAGGGTAGTAGAGGGTAGATGCAGTCCAGCT17300.10607574912165602No Hit
ATATAAAACCGAGAGTGAAATTTAGAAATCCAAGCCCAAAGAGGACTGGA17190.10540127904053566No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16630.10196761317301385No Hit
CCCTTAAGATGTTAGTGTGATTTCTACCATAACAAAGACCCAGGTCAGTC16530.10135445855381353No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA16390.10049604208693305No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG100.0070288396145.4295145
TGGACCG151.2157376E-4145.42949145
TTATACG351.0913936E-11145.42949145
CGCCGTG151.2157376E-4145.42949145
AGCGAAA5050.0123.519461
TTGCTCG203.821272E-4109.07212145
ATGTGCG203.821272E-4109.07212145
CCGACGG404.7184585E-9109.07212145
CACGTGG203.821272E-4109.07212145
ATCGCCG203.821272E-4109.07212145
CCGCACG404.7184585E-9109.07212145
ATACACG501.9099389E-10101.80065145
TGACGCG650.0100.68195145
ATTCCCG301.5764386E-596.952995145
GCGAAAG6900.090.3714142
CAGGTAC1650.087.8768543
CCCGAGG259.2781574E-487.2577145
CGAAAGC7450.083.689443
TTTGACG300.001913379172.714745145
AGGTACT2150.070.801544
Produced by FastQC (version 0.11.5)