FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635841
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT7080.11134859186494737No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT203.875745E-4108.676974
ACGCGTA259.4131834E-486.934745
AGCGAAA1000.072.502641
TAGATCG501.9774679E-458.189907145
TGGTCGA400.005972130754.55304145
GTACGCG450.00965714148.3084753
CGCGTAA450.00966614948.2970736
GCGAAAG1703.8198777E-1142.6452522
CGAAAGC1755.2750693E-1141.407263
CTCTACG1354.9661685E-737.593961
GTAGAAA4300.037.094371
TATAGTA2550.036.9592172
GTATAGT2650.035.567331
GTATACC1102.4447788E-432.9324154
TATACCC2009.0058165E-932.6005255
AGCTATC2900.032.485849
GTATATA1402.7444294E-531.0725571
TAGTACT3750.030.912564
CGCTGCG1652.3245811E-630.858282145
GCATTAA3100.030.4043331
Produced by FastQC (version 0.11.5)