FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127919
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC2330.1821465145912648No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC1580.12351566225502077No Hit
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC1570.12273391755720417No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC1540.1203886834637544No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC1490.11647995997467148No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT1310.1024085554139729No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAA300.001942697172.397931
ACTATAC502.0175906E-457.9183463
TAATGGT400.006063227554.3196874
CCTACGG400.006072622754.2984433
ATATAAC450.00967375848.2652853
GTACTCC700.001060509141.3702471
GTCTTAG700.001060509141.3702471
GCAGGAC2200.039.489783
ATTATCT800.002044077336.1989633
GGACAGT1854.1636667E-935.234396
CTATACA1051.8557726E-434.4886934
TATAACT900.00363456232.1894454
AGTAGAA900.003641535832.1768571
ATCATAC900.003641535832.1768573
GTAGAAA1153.172921E-431.477361
GACAGTG2003.24575E-728.9705037
TTAGAGT1000.006085100628.9705034
TAGAAAC1000.006096749628.9591733
GAGTGAG1855.810065E-627.4045287
AGAGTGA1658.420401E-526.3368196