FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489472
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT63310.4250499505865165No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT38760.26022644265887507No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA30530.20497196321918104No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA21610.14508496970738624No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG21490.14427931508615133No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG20980.140855282945903No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA19810.13300015038886262No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT19500.13091887595067248No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG19430.1304489107549521No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA18460.12393653589996992No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT17540.1177598504705023No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC17260.11587998968762085No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA17110.11487292141107722No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT16170.10856196021140378No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA16120.10822627078588923No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC15920.10688351308383104No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT15860.1064806857732136No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT15430.10359375671378851No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC15250.10238527478193615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3300.074.6683351
TGACGCG607.8645826E-872.72382145
CGAAAGC4500.054.7383883
GTAGAAA12350.052.2269331
GCGAAAG4750.051.8661272
CGACCAA2300.047.248615
GACAGTG24700.044.58487
GGACAGT24850.042.857926
CCGACCA2650.041.0054744
CAAGGAC26400.037.321633
AGTAGAA11350.033.841651
ACAAGGA31200.032.746362
TAGAAAC20650.032.633332
AGGACAG34000.032.6015435
TGTCTCG1952.5392546E-729.835415145
AAGGACA32650.029.7315864
AACAAGG37500.029.37541
GTATATA3550.028.5805871
GAAACAA22900.028.1547034
CAGTGGG36750.027.9962839
Produced by FastQC (version 0.11.5)