FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890579
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG100.007030117145.41696145
AGCGAAA3350.086.596491
GGTACGG451.2302935E-680.582851
GCGAAAG4800.060.423552
CGAAAGC5050.057.412933
CGGAGTA651.0949929E-555.7567835
GCGATTA701.696946E-551.7799726
GATTACG701.6975104E-551.7770658
ATCGCGG450.00964515348.325268
TCGCGGA450.00964515348.325269
CGATTAC855.3462827E-542.639947
AGCGATT855.3480555E-542.6375435
ATACTCC2050.042.4295545
TTATACC1451.933222E-839.986584
GATAACC2550.039.7972767
TATAGCG1105.2595315E-639.549962
ATTACGA951.0298117E-438.1515249
TAGATCG800.002007989736.35424145
TATACAG3400.036.241915
GTACATA1251.2619992E-534.811791
Produced by FastQC (version 0.11.5)