FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_B01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_B01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046171
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT24870.1215440938220706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4300.050.5947571
CGAAAGC4350.049.9826433
GCGAAAG4900.044.3875352
ACGGGCG1651.3260433E-939.66845145
GTAGAAA13600.039.458951
CTATACG1153.169695E-431.5107963
CTCGGCG950.004657153530.621264145
AGTAGAA11950.029.7358861
ATGAACG4450.029.3127986
CCGACGG1000.005984429729.090197145
TATAAGG10600.028.7262762
TAGTACT7350.027.6054124
TACCTAT8900.026.8700667
TAACGAC2705.5915734E-926.8425313
CCATCGG4400.026.3525529
ATACACG1409.818613E-425.97339145
TATACGT7000.025.8800744
TCCCGCG1701.01026024E-425.667822145
GTATAGC12150.025.6652131
CACATCG2351.296039E-624.757614145
Produced by FastQC (version 0.11.5)