FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832361
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT42790.2335238525596212No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC38870.2121306882213712No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT29820.16274085728740134No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT26920.14691428162900214No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG26610.1452224752655181No Hit
GATAACATACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAATGAATA26380.14396726409261057No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC26030.14205716013383826No Hit
CCATAATAAATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCC25200.13752748503160678No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC24030.13114228036942502No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG19290.10527401532776566No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC18880.10303646497606095No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT18540.10118093541611069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG554.3028194E-879.33688145
TGACGCG753.6830534E-758.180378145
AGCGAAA2900.057.4992521
TATACTG13700.056.5970765
CGGATCG450.00952286448.48365145
CCGTATA752.5050107E-548.483646145
GCGAAAG4150.047.168061
TCGCCGG1109.724863E-846.27985145
GCTATAT4250.046.0582281
CGACGAG803.6679874E-545.453423145
ATATACT15950.044.9809884
CGTTTAT4200.044.880371
AATATAC16900.042.455953
CATTAAG12950.041.982142
TTATACG1806.91216E-1140.40304145
AAGATGT13100.039.2772946
AGATGTA13050.038.872467
CTATATA5450.038.5722162
TACTGTG30050.036.1743977
GTAATAT20600.035.5456541
Produced by FastQC (version 0.11.5)