FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650888
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACACATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGCGAACA28100.43171789923919324No Hit
TTACAAAGGAGTACCATATGATGGAGACAGAACCATCTTGAGAAAATTGT20300.31188161404112535No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG18580.28545617679232066No Hit
GGTTACTTGTTGTTAGGAAGCAAAATCAAACTGATTACAATTACAAAGGA15400.23659984513464682No Hit
ACTCTGGGTGGTCTGTTCATCATGGCTTCCTGCTGCTGCTTCATCCGGGT14450.22200440014257444No Hit
GAATAAGCCTGGCCTCAGTTTTTCTGACGCGACAGAGGTTTTGGGCACTC14440.2218507638795No Hit
GTCATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGCAGGCCCAGGTG14380.22092894630105334No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC14240.21877803861801107No Hit
GTGTAATACAGAAGCATGAGTCAGGAGTAAATTTGTTCCCTGACACTCAT13040.2003416870490776No Hit
CACTTATGTTCGCATTTCTTTAAAATGAAGGAAACACCCAGTAATATGTG12340.18958714863386636No Hit
GTTCAGGAGGACCCAGTTGTGCAGAAGCAGTGGGGGCCAGCTGGTGTCGC12310.189126239844643No Hit
GGATAATCCCGCGCCACCTGCAACTCGCCATCCGCAACGACGAGGAGCTC12180.18712896842467522No Hit
GTCTAAGGGAAAGCCGTGACCCCGGGGTCCAGCAGGTGCCTGCCCTGTGC12020.18467078821548408No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT11440.1757598849571662No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA11070.17007534322341172No Hit
GGCCTGCAGTTCCCGGTGGGCCGAGTTCACAGACTGCTGCGCAAAGGTAA11000.1689998893818906No Hit
GTGTTGGAGTACCTGACCGCCGAGATCCTGGAGTTGGCTGGCAATGCCGC10880.16715625422499722No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10790.1657735278573272No Hit
CTACAAGGACAGTCATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGC10150.15594080702056268No Hit
CCCCAAGAGATCGCCGCAGACTCCCATGACATCGAGTATATTGAAAAATA10040.15425080812674377No Hit
GTAATATGTGTAATACAGAAGCATGAGTCAGGAGTAAATTTGTTCCCTGA9750.14979535649758485No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG9750.14979535649758485No Hit
CTTCTGTATTACACATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGC9730.14948808397143595No Hit
ATATTTTTCTTAGCCCAATAGCCGTTGTGTTTTCAGGTTTTGGATATCAG9520.14626172244687258No Hit
CTTATGTTCGCATTTCTTTAAAATGAAGGAAACACCCAGTAATATGTGTA9510.14610808618379814No Hit
GAAAAATACTGCGGGGTGGCTGTGGAAAGAGCCTTTGCTGGGGAAGCCAG9510.14610808618379814No Hit
ACCTCATTCAGAACTACAAGGACAGTCATGAGGACAGACTGCAGGAGGCC9500.14595444992072368No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA9500.14595444992072368No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA9290.14272808839616033No Hit
TTTCAAAGGTGGAGTAACTTTCCGTCTTGGAAGAGTGCTTCTGATGACAC9110.1399626356608203No Hit
GTATACACACTTATGTTCGCATTTCTTTAAAATGAAGGAAACACCCAGTA8960.1376580917147036No Hit
GTACCATATGATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCT8760.1345853664532147No Hit
GATTACAATTACAAAGGAGTACCATATGATGGAGACAGAACCATCTTGAG8640.13274173129632133No Hit
CTCCTGTCTTGTCTGGGCAGTTCAGGAGGACCCAGTTGTGCAGAAGCAGT8620.13243445877017243No Hit
GTACTTGGGGACCTTGCTGTAGTCTTCAGAGTGGACGTGCCGGCACAAGC8580.13181991371787466No Hit
CTCCCATGACATCGAGTATATTGAAAAATACTGCGGGGTGGCTGTGGAAA8430.12951536977175798No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA8370.12859355219331128No Hit
CCTCAGTACTTGGGGACCTTGCTGTAGTCTTCAGAGTGGACGTGCCGGCA8310.1276717346148646No Hit
GGGATGGAGAGAGGTGTTTGGCGGAGACGGAGGCGTGAGGTAGGGGCCCA8260.12690355329949238No Hit
TCTTCATGCAGCCCTCATGGTACACGGGCCAGTACTCGGGGCTGTTCCCG8220.1262890082471946No Hit
GGCCAGTACTCGGGGCTGTTCCCGCTCTCGGTCCTGTTGCTGTCGAAAGC8140.12505991814259904No Hit
ACTTTGGTCAGCACCCTCGAGGTGTTTTGTGGGTGGGGCGAGGGAGACCA8080.12413810056415236No Hit
AATCCATTCCTTCTGGGAAAAGCGGCTTTCAAATAAAACAGGCAAAAGAA8040.12352355551185457No Hit
CATCGTGACCAACCTCATTCAGAACTACAAGGACAGTCATGAGGACAGAC7980.1226017379334079No Hit
ACATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGCGAACATAAGTGT7860.12075810277651455No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA7760.11922174014577008No Hit
GTTACGCAACATGAGTGTCAGGGAACAAATTTACTCCTGACTCATGCTTC7760.11922174014577008No Hit
TACTTGTTGTTAGGAAGCAAAATCAAACTGATTACAATTACAAAGGAGTA7750.11906810388269565No Hit
GCTCCTAAGCATGTAACTCATGACTAAAGACACTGCACGGTTACGCAACA7530.11568810609505784No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC7500.11522719730583449No Hit
TTGCTGTAGTCTTCAGAGTGGACGTGCCGGCACAAGCAGATGGACAGGAG7490.11507356104276004No Hit
ACTAAAGACACTGCACGGTTACGCAACATGAGTGTCAGGGAACAAATTTA7430.11415174346431337No Hit
CTAGAAGCCCATCTGTGGCATTCTTAAGATTTCATTTATCTGACAGAGAA7350.11292265335971781No Hit
AAATCAAACTGATTACAATTACAAAGGAGTACCATATGATGGAGACAGAA7320.11246174457049446No Hit
CATAAGTGTGTATACAAAAATATCTGATATCCAAAACCTGAAAACACAAC7180.11031083688745223No Hit
GCCTAGGATGATGCCCACGTTCTCCTGCAGCCAGGCCTGCACCTTCTTCA7150.10984992809822888No Hit
ATATGATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCTCCTAA7150.10984992809822888No Hit
CATAGAGACAGAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTG7100.10908174678285665No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA7050.10831356546748441No Hit
CTCTAGGCACCTTGTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAAC7050.10831356546748441No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT7030.10800629294133553No Hit
ACATAAGTGTGTATACAAAAATATCTGATATCCAAAACCTGAAAACACAA6990.10739174788903774No Hit
GTACACGGGCCAGTACTCGGGGCTGTTCCCGCTCTCGGTCCTGTTGCTGT6880.10570174899521884No Hit
CATATTACTGGGTGTTTCCTTCATTTTAAAGAAATGCGAACATAAGTGTG6850.10524084020599551No Hit
CATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGCAGGCCCAGGTGAA6830.1049335676798466No Hit
GTGCAGGCCCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGAC6830.1049335676798466No Hit
ATCCTGGAGTTGGCTGGCAATGCCGCTCGTGACAACAAGAAGACCAGGAT6790.10431902262754882No Hit
TCTTGGAAGAGTGCTTCTGATGACACAGGGCCAACATATTTTTCTTAGCC6770.10401175010139993No Hit
CTGCAAATCCATTCCTTCTGGGAAAAGCGGCTTTCAAATAAAACAGGCAA6770.10401175010139993No Hit
CTCCTGAACTGCCCAGACAAGACAGGAGAGTGAAGGCCCCGGGAATGACA6730.10339720504910214No Hit
GTGCGGGCCAAGGCCAAGTCTCGGTCCTCCCGCGCGGGCCTGCAGTTCCC6700.10293629625987881No Hit
AAGCAGGCCTGCAAATCCATTCCTTCTGGGAAAAGCGGCTTTCAAATAAA6690.10278265999680437No Hit
GATGGAGACAGAACCATCTTGAGAAAATTGTCTGAACGGCTCCTAAGCAT6630.1018608424183577No Hit
CTCTTGGGGAGGTGGGTGGCGAGGGGCCCGCCGAGTCCGGGGCCAATAAA6600.10139993362913435No Hit
ATGTAGTCTCGGGGACCTTCCGGATGACTCGGGGGGCAGCCAGGAGAGCT6600.10139993362913435No Hit
GATGGAGAGAGGTGTTTGGCGGAGACGGAGGCGTGAGGTAGGGGCCCAAG6510.10001720726146433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG306.366463E-10145.44295145
CGAAGCG151.2149558E-4145.44295145
CCTACCG100.0070259306145.44293145
TTTCCGG100.0070259306145.44293145
CCGTATG100.0070259306145.44293145
TAACGTT151.2307083E-4144.97349
ATAACGT151.2307083E-4144.97348
TATGCGT100.0070958165144.962274
AGCGAAA4700.0134.167191
TAGATCG256.3658827E-6116.354355145
ATTATCG256.3658827E-6116.354355145
TTGTTCG256.3658827E-6116.354355145
CTGACGG404.7111826E-9109.0822145
TTATACG352.7109309E-7103.88782145
CACATCG650.0100.69126145
GCGAAAG6350.099.297222
TTATAGG353.4447483E-582.816483
CCTGTCG801.8189894E-1281.811646145
AATAACG451.2337459E-680.540787
CATCTGG607.8593075E-872.72147145
Produced by FastQC (version 0.11.5)