FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200061
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG604.8712324E-448.48181145
TAGGTCG604.954196E-448.3160745
AGCGAAA3500.043.508051
CGAAAGC3650.041.7009123
CGACGAG700.001040897641.555836145
CAATTCG4400.037.8841939
CAGCTCG5400.037.579179
TATACAG6650.037.054435
CCGACGG1000.005984569429.089087145
TATATAC10750.028.9920643
GTATATA9850.027.9747161
GCTATAT1658.409358E-526.3685111
GTATTAG2507.819472E-826.1048281
TTACGCA3657.2759576E-1225.814854
GCGAAAG6000.025.3744012
GTATTAC4650.024.950851
GGTCGTT3650.023.8271057
GTTACGC4353.6379788E-1223.3269463
TATACTG8100.023.2632945
TACGCAG4750.022.8865625
Produced by FastQC (version 0.11.5)