FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558529
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT39240.15336937748213916No Hit
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC33290.13011382712488306No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG27650.10806991048371935No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC27610.10791357064938487No Hit
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC26220.10248076140626117No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC25990.10158180735883784No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG353.3859957E-583.113625145
CGTTGCG353.3859957E-583.113625145
CGGTTCG6100.045.1690521
AGGACCG3300.041.87164145
TGACGCG1053.7460268E-641.55681145
AGCGAAA6050.037.152971
GCATTAA16050.037.0446971
CGAAAGC6300.036.8216482
GGTCGCG1051.8211425E-434.630672145
GTTTACG850.002701530734.22326145
ACTAACG850.002701530734.22326145
GACGCTA850.002701530734.22326145
CATTAAG17200.033.7174952
CTACTCG1956.7939254E-933.565117145
GCGAAAG7050.032.911531
TTCGCAG8650.032.6755874
TGGTGCG10050.032.4517677
ACGGGCG2506.730261E-1131.998743145
GGTTCGC9250.031.3481622
GTAGAAA15200.031.0068021
Produced by FastQC (version 0.11.5)