FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383466
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA28720.12049678912977992No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT28260.1185668266297904No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG26570.11147631222765501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG202.0993102E-6145.44014145
CCGACGG755.4569682E-1177.56808145
TGACGCG702.748493E-972.72007145
ACGGGCG954.4383341E-1061.237957145
GACCGTC753.7742757E-757.9758347
TAGTTCG604.8730848E-448.480045145
GCTACCG1551.4551915E-1146.916172145
AGCGAAA3950.044.0470661
TTATACG1359.998985E-943.093376145
CAATTCG4500.041.8723149
CCTTATA11150.038.9988332
CGAAAGC4650.037.4014053
ATACCTA12400.034.4794246
TACCTAT12600.033.3591087
TATACGT4350.033.3159454
ACTGATC8500.031.5463358
TAATACC15450.030.4857084
TAGACAG14500.030.4847345
GTATTAC14450.030.101371
GTAATAC16400.030.046563
Produced by FastQC (version 0.11.5)