FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194536
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA135940.6194475734278225No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT92150.41990653149458473No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT90300.411476503461324No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG81870.37306291626111393No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT77640.3537877710823609No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG68550.31236671442163627No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA67320.30676188497249535No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC59980.2733151791540444No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC59520.2712190640755039No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG54840.24989337153730903No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG52900.24105323403215986No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG52800.24059755684117282No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA51140.23303331547078746No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50500.2301169814484702No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA44930.20473576191049042No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG44390.20227510507916024No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG44390.20227510507916024No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG43200.19685254650641412No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG41630.18969841460791712No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG39980.18217974095663048No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG39850.1815873606083473No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG39500.17999249043989254No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC39270.17894443290062229No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG38830.17693945326027916No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC38500.17543571853002182No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC37130.16919294101349897No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG37040.1687828315416106No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA36350.16563865892379984No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA36290.1653652526092076No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT35090.15989712631736278No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA34150.1556137607220843No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC33360.15201391091328648No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA33340.15192277547508903No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC32830.149598821801055No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG32130.1464090814641455No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG31910.14540659164397396No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG31840.145087617610283No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC31520.14362945059912438No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT31390.14303707025084117No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA30170.1374778085207989No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC30050.13693099589161445No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT29160.13287546889182952No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA28410.1294578899594265No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCACCCGAATTCTTTTGGTCG27740.12640485277981314No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA27670.12608587874612218No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA27020.12312397700470624No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC25950.1182482310611446No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA25760.11738244439826916No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG25180.11473951669054416No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT24410.11123080231994371No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT24200.11027388021887087No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG24090.10977263530878509No Hit
AAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGAT23830.10858787461221871No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC23550.10731197847745491No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT23380.10653732725277688No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA23100.1052614311180131No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA22950.1045779153315325No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG22910.10439564445513767No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT22900.10435007673603895No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT22360.10188941990470879No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT22350.10184385218561008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA17700.0113.06051
TTATACG203.821377E-4109.07265145
GTCGTTA203.821377E-4109.07265145
TGGACCG900.0105.03292145
CCGACGG352.7145143E-7103.878716145
TGACGCG501.9099389E-10101.80115145
TCGTTCG502.2237145E-887.258125145
AACGACG259.2784094E-487.258125145
TATCGCG259.2784094E-487.258125145
GCAGGTA13600.083.694362
CAGGTAC14750.078.1360553
ATTAGCG755.4569682E-1177.562775145
AGCGAAA3750.075.406441
GTAGAAA27400.072.7641752
TCGATCG300.00191343172.7151145
GTATCCG300.00191343172.7151145
TCCAACG406.56785E-572.715096145
CGAAAGC4000.072.48473
GGTACTG16700.069.4321755
ACTGATC17100.069.079438
Produced by FastQC (version 0.11.5)