FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968319
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG88820.2992265992974475No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA80990.27284803284283127No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT68370.2303323867818789No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT62630.21099484253545528No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA60640.2042907113420087No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG57920.19512727574091598No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC55570.18721033689438366No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG51110.17218499763670952No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC50780.17107325728804754No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG50440.16992782783791094No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA49450.166592606791925No Hit
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT48690.16403223507985495No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG48050.1618761325854802No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT47790.16100021594714045No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA47540.16015798841027531No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT47140.15881042435129109No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG45690.15392550463747326No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA43480.1464802132115854No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA42270.14240383193315811No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT41400.1394728801048674No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG41380.13940550190191822No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG40500.1364408609721529No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT39870.13431844757925276No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC38130.1284565439226714No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG37010.12468336455751555No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG36850.12414433893392186No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA36070.12151658901890262No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT36030.1213818326130042No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA35750.12043853777171523No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT35290.11888883910388338No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT34140.11501459243430372No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA34120.11494721423135452No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT33860.11407129759301475No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT33770.11376809567974333No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT33300.11218470791043685No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC33050.11134248037357171No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG32950.11100558935882565No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG32210.10851259584970484No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA31580.10639018245680468No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT31190.10507630749929506No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30990.10440252546980294No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATG30760.10362767613588701No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30520.10281913770049647No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA30300.10207797746805515No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT29810.10042721149579947No Hit
ACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGA29690.1000229422781042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG100.007025365145.45555145
GTAACCG100.007025365145.45555145
GTACGAT203.872834E-4108.707496
GTACCCG406.0072307E-790.90972145
AGTAGAA11100.086.892521
TCCCGCG353.3853103E-583.117455145
AGTTCCG801.8189894E-1281.81875145
GCAGGTA5050.077.524492
AGCGAAA2150.074.205811
TGACGCG300.001912175272.727776145
TACATCG1300.072.727776145
CACATCG851.6552804E-1068.44967145
CAGGTAC5850.066.904813
TCTCGAG1900.065.07222145
CTACTCG451.1758492E-464.64691145
GTTGGCG807.896233E-963.636803145
ATCATAG1850.062.899696145
ATTAGCG350.0035231462.338093145
ACGGCCG350.0035231462.338093145
ACCGTAG350.0035231462.338093145
Produced by FastQC (version 0.11.5)