FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213935
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA48370.39845626001392165No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG26580.21895735768389574No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC24980.20577708032143402No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG21580.17776899092620282No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG18810.15495063574244092No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC18560.15289121740455625No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG18090.14901951092933313No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT15870.13073187608891743No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA14990.12348272353956348No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC14040.11565693385560182No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC13980.1151626734545095No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT13880.11433890611935565No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA13220.10890204170734019No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT12810.10552459563320936No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC12430.1023942797596247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC400.006058098354.3593339
TAAGACG553.2258275E-452.7055664
TCATTCG803.6647634E-545.45864145
CGGCATA855.2416308E-542.784603145
AGCGAAA3450.037.8323361
CTAGTAC2252.0008883E-1135.432773
ACCATCG9350.034.8815578
AACGGAT850.00274609234.1078157
ATGAACG7450.034.0491876
TAGTACT4400.032.940984
TTAGCGT900.003635858232.2089584
CGAAAGC4100.031.8187893
AGTGATC24450.031.7175668
GTAGAAA10750.031.702911
GGCTTAT17450.030.7500631
TGAACGT8500.029.8443417
CCATCGG10250.029.698769
GAACGTC8550.029.6685898
GACCATC13350.029.3173927
TACTCCG1255.163182E-428.9916447
Produced by FastQC (version 0.11.5)