FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363078
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA193460.8186780123212183No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC90140.3814516490780245No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG86090.3643129850136136No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG74640.31585923105373587No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG69990.29618150564644924No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG66840.28285143359635184No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT61460.2600845168885665No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC55740.2358787987531516No Hit
GGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGA54510.23067372300025643No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA49990.2115461275505929No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC47970.20299795436291143No Hit
CAATAATTGGTGTCCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTAT45020.1905142360937726No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC43440.18382804122419996No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT41710.17650708101890838No Hit
ACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGAGATAA41430.1753221857255664No Hit
GTCTTAGCCAAGTCACTTCACCACTTTTTGACTGTTTACTCATTTGCCAA37160.15725253250210108No Hit
GTATTTCCACAAAACCGTCTCAGATTTGATTGACCAGAAGGTGTATGAGC36960.1564061787211425No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC36270.15348625817683548No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA35960.1521744098163497No Hit
GTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAACCTCTGCTCCAAGG35620.15073560838872013No Hit
TCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAAT33960.14371087200676405No Hit
AAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACT32830.13892897314434816No Hit
GTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAGTACACTATAG32760.13863274932101266No Hit
GCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAA32550.1377440778510062No Hit
GCTTAACAGCTATCATTTTAGGATATAAAAAGCGGTTTTCTTTTTATCTT31280.13236973134191932No Hit
GCATATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGC31140.1317772836952483No Hit
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC31040.13135410680476906No Hit
GGCATATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGG30570.1293651754195164No Hit
AACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGC30150.12758783247950342No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC30060.12720697327807207No Hit
CCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAG29670.12555658340520287No Hit
ATTTTTATTAGACATGAGGTCTTAGCCAAGTCACTTCACCACTTTTTGAC29050.12293288668423133No Hit
GTGTATTCTTGAAGAACCTTTCAGTCAACTTGGTCCAGCTGTTCTCATAG28790.1218326267689852No Hit
AGTTGTAGTAGGACTCAAACCTCTGCTCCAAGGAAGGCCCTCCACTGACT28560.12085931992088285No Hit
CTTTTTGACTGTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAG28330.1198860130727805No Hit
GGATCTGGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCC28030.11861648240134265No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC27840.11781244630943202No Hit
GTGTATGAGATCCAGGACATCTATGAGAACAGCTGGACCAAGTTGACTGA27590.11675450408323382No Hit
GTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTA27570.11666986870513796No Hit
ATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTCCA26640.11273432362368065No Hit
CCACTGACTTTGGCATATATGTGCCTGTAGTATAATTCTTTGTACAGAAT26310.11133783988509902No Hit
AGTAGAAACAAGGCATTGGGTTACATAGAACTGATTTTTAGCATGTGCTC26240.11104161606176352No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT26160.1107030745493801No Hit
GTAGAAACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAG25890.10956049694508603No Hit
CCACAAAACCGTCTCAGATTTGATTGACCAGAAGGTGTATGAGCTGCAGG25790.10913732005460675No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG25560.1081640132065044No Hit
GCTCCAAGGAAGGCCCTCCACTGACTTTGGCATATATGTGCCTGTAGTAT25480.10782547169412098No Hit
GGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTA25170.10651362333363519No Hit
ACTCTGGATCATGGAGATGACTACTGGAAGGGTCCCGTGTCCCCGGGCTC24700.10452469194838258No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG24150.10219721905074652No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG24130.10211258367265068No Hit
CCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTATGAACCATGTTTAA24030.1016894067821714No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG100.0070248432145.45854145
AGCGAAA5900.078.670541
TCGTTCG651.5261321E-978.32383145
CCGACGG502.264338E-672.72926145
AGTAGAA31350.060.379161
GCGAAAG8050.058.5414772
CGGCATA1153.8198777E-1156.918556145
CGAAAGC8200.056.5708583
GCTACCG902.0007064E-856.56721145
ACCGCCG651.0738302E-555.94559145
TGACGCG651.0738302E-555.94559145
TATGCCG553.170097E-452.894012145
GTAGAAA39900.047.9860731
GCTTATG22200.047.681082
ATGAACG22250.047.227086
TTAAGAT13350.046.6852264
CTTATGA22900.045.5778353
CGTATCG657.2253705E-444.756474145
TAAGATG14350.044.439135
TATACGT7400.043.090674
Produced by FastQC (version 0.11.5)