FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16447421
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2276591.384162295109975No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG418260.25430126704970946No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA413010.25110927725386245No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA392960.23891891622400863No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA349410.21244060086988711No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT283920.17262280815940687No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG258680.1572769372170871No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG234830.14277618357309635No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC225600.13716436151296912No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC224020.13620372458393326No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT205500.12494360058029766No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT200200.12172121088163305No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT197470.12006137618779261No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG194580.11830426180493586No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG182330.11085628561462615No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC180990.1100415682191147No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT176000.10700765791791916No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG169940.10332318969642719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG23300.0101.2201167
CGTATAG20300.079.3626565
CGCTATA16600.063.8214954
ATACGCG6050.062.3699578
TATATCG27000.060.472537
GGTATAG35750.056.4323275
GGTATAC24300.056.1449665
GTATAGG44550.054.89966
TATAGGG45600.054.425287
CGATATA23200.051.6081354
TATACCG31650.051.1293877
GGGTATA39500.050.5193524
CAGGTAC82850.050.01563
CAATTCG157300.049.7605749
ACGTATA26000.049.6785434
TATAGGC32200.049.5799647
CTATAGG33400.049.324926
AGTAGAA181900.049.2725941
GCAGGTA87150.048.709762
GTTATAG59550.046.5522655
Produced by FastQC (version 0.11.5)