FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608426
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1431273.105767565758895No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT784921.703227956790453No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA665481.4440505283148737No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC438050.9505414646996609No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT429330.9316196028752549No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG406770.8826657952194524No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG392720.8521781623487065No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA371310.8057197837179114No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA339640.7369978383074829No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA331920.7202459147656923No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG274780.5962556412970502No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG265480.5760752152687274No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG263390.5715400442580612No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG254370.5519672009488706No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG250920.5444809138738476No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG239990.5207634884448616No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC217050.4709851042416652No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG195660.42457012437652253No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA195620.4244833268452179No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA190230.4127873595019211No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT189270.41070421875061025No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT184070.3994205396810104No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA183930.3991167483214443No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG178080.3864226093681443No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC174180.37795985006594446No Hit
ACTTAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAA173490.3764625926509398No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT168850.3663940790196045No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT160320.3478845054688955No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT159830.3468212357104139No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC156650.3399208319716971No Hit
GGATACACCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGG150460.32648891400230795No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA146960.3188941300131542No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC144240.3129918978844404No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG142460.30912940774138503No Hit
ACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATG141310.3066339787163773No Hit
CCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGGAATCGTT141200.30639528550528966No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG139760.3032705743783235No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA137840.299104292875702No Hit
CTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC136130.29539369841242974No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA133900.2905547360421975No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA133000.28860179158784366No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC132780.2881244051656683No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC130350.28285145513891297No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA120450.2613690661410208No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT119440.2591774284755793No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA115780.2512354543612071No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC113160.2455502160607548No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG110900.2406461555420441No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACATCTG110740.24029896541682563No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA107940.2342231382255026No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA107190.23259568451354107No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC106970.2321182980913657No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG105760.2294926727694011No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAGCACCACATTC102450.22231017705394424No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG100330.21770990789479966No Hit
GGATACACCATGGATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGA97980.21261055293065353No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA97430.21141708687521507No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATAGGAATCGTTCTA96640.20970283563194894No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT94990.20612243746563358No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT94100.2041911923941059No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG93290.2024335423851875No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTT92830.20143537077518442No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA92390.20048059793083364No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA91070.19761627939778137No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC90200.19572843309190602No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA89840.19494725531016446No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT89540.19429627382537984No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT88660.19238672813667834No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT85570.18568161884339685No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC82070.17808683485424306No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG81920.17776134411185077No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT80690.17509232002423386No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC79640.17281388482748775No Hit
CCTAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAA79230.17192421013161543No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG78990.17140342494378774No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC77940.1691249897470416No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT77720.16864760332486625No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT77720.16864760332486625No Hit
TCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG77620.16843060949660468No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG77520.16821361566834314No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC77260.16764943171486318No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT75540.16391713786876475No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG75040.16283216872745704No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT73620.15975085636614322No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG72370.15703843351287403No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTGCGCAAAATGCCA71560.15528078350395558No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC70580.15315424398699254No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAA70260.1524598637365556No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC68920.149552146437851No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA68080.14772939828045412No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC67860.14725201185827871No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG67710.14692652111588642No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA67230.14588495074023103No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA66950.14527736802109875No Hit
GACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGA66060.14334612294957105No Hit
ATTCCCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT65650.14245644825369877No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA65060.1411761846669557No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA64410.13976572478325572No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT63680.1381816698369465No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA63450.13768258403194497No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG63310.13737879267237882No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC63070.13685800748455113No Hit
GAACTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCC62660.13596833278867881No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATT62360.13531735130389422No Hit
GTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATG61370.133169112404105No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT60740.13180205128605732No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC60720.131758652520405No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA59520.1291547265812666No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC58990.12800465929148042No Hit
GACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCC58070.12600831607147428No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC56810.12327419383537892No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA56380.12234112037385432No Hit
ATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG56310.12218922469407124No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATTCCTGGA56140.12182033518602664No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA55900.12129954999819895No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA55730.12093066049015434No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT54780.11886921912166976No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT54650.11858712714492975No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA54050.11728516417536053No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA54050.11728516417536053No Hit
GTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCA53820.11678607837035898No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC53710.1165473851592713No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT53170.115375618486659No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG53120.11526712157252823No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT52850.11468123823622207No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC52750.11446424440796055No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA52400.11370476600904517No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA52150.11316228143839133No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC50990.11064515303055751No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC50700.11001587092859906No Hit
GAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTG50410.1093865888266406No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50170.1088658036388129No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC49370.1071298530127206No Hit
TCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTG49140.10663076720771908No Hit
GCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTTTTCCCTA47680.10346265731510064No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA47460.10298527089292527No Hit
CTTCAATGGTGGAACAGATCTTATGATCTCAGAGAACTCTTCTTTCTTGA47050.10209559619705295No Hit
GAATGACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGT46930.10183520360313911No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGG46740.1014229153294422No Hit
GGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACAC46650.10122762088400682No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA46550.10101062705574528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA116800.0121.596091
AGCGAAA12100.0107.903851
GAGCGAA1700.0106.669651
GCAGGTA21950.0104.723112
GGTCAAA37350.0101.180511
CAGGTAC23600.098.0126953
ACCGGAG1450.095.014363
GTCAAAT42600.088.513912
CCGGAGA1650.087.890174
GTAGAAA164350.086.301222
TAGAAAC167150.084.809413
GCGAAAG15800.083.069052
TACGGAG1050.082.867874
AGAAACA171700.082.644664
CCGCACG1850.082.29723145
GGTACTG28500.080.905215
AGCAGGC29300.080.677723
AAATATA47850.079.707295
ACTGATC29500.078.8923348
CGAAAGC16850.078.750783
Produced by FastQC (version 0.11.5)