FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961033
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC69100.23336450488731464No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT64430.2175929819086785No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC47170.1593025136835692No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC46070.15558759392414742No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA42160.14238274277929358No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG41620.14055905489739562No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT40040.1352230792429534No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT37900.12799587171098736No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC35530.11999190822932401No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG35310.11924892427743966No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT34600.11685111243272196No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG31930.10783398901667088No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG31560.10658442509759262No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA30600.1033423133075518No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT29880.10091072946502115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7250.065.026591
TACGACG651.0925072E-555.783563
CAATCGC400.006052371654.374275145
GCGAAAG8850.053.265872
CGAAAGC9350.048.8628233
ACGACGT803.7318918E-545.3218464
GCATTAA27350.041.104561
AGATGTA29350.036.5632257
TCGTTCG1102.4374874E-432.95411145
GTAGAAA34450.032.6330831
GGTCGTT3050.030.9054037
CATTAAG37700.030.2021052
GTCTAGT18500.028.6198121
TAAGATG43500.028.5058785
ACCGTGC28100.028.1252548
CATTGCG1555.491858E-528.0736262
TCCACCG30300.025.6075945
CCACCGT30900.025.3450346
GAGCGAA3501.2914825E-1024.8673111
TAGTACT36750.024.0729924
Produced by FastQC (version 0.11.5)