FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581222
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC35960.13931385986947267No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA28500.11041281997441522No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT27500.1065386859402252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5850.059.5118561
GCGAAAG9250.037.6306652
CGAAAGC9850.034.6002163
GTAGAAA28950.034.573921
CAATTCG7250.030.9996419
AGTAGAA32450.028.6097091
AGTACTG23800.028.0297745
TAGTACG1907.152117E-626.7148784
TATATCG1100.00966668926.367935
GTATTAC13950.025.9964431
TTAGTAC15800.025.2419433
GAGCGAA4051.8189894E-1225.0721261
TTATACT28100.025.0307664
TATACTG30500.024.7253385
GTATAAA11150.024.7187731
CCTTATA24350.024.4206542
GATTAGC10150.024.295791
CACCGTA2101.5555115E-524.1682627
CTTATAT10350.023.8221512
TAGTACT34600.023.6816024
Produced by FastQC (version 0.11.5)