FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295680
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT33300.10104136323914943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8000.053.4948961
GCGAAAG9850.044.172632
CGAAAGC10300.043.649533
AGTACTG23750.032.0580865
CGAATTG1050.00770457627.6237495
TAGTACT32500.026.99694
GTAGAAA24250.023.929221
ACCGTCA3652.2009772E-1023.8367778
CTAGTAC25900.023.5182293
GCGCTAT3203.3740434E-822.6673281
GACCGTC3854.292815E-1022.5985037
GTACTGT24550.022.4478136
GTATACC11100.022.2109854
GTACTGA15700.021.7075146
CGCTATT3457.4993295E-821.0193442
TTAGTAC21700.020.3842723
TTATACT14600.020.3630524
GCATTAA21400.020.3370441
GTCTAGT9050.020.0374181
GCTATTG17450.019.9436668
Produced by FastQC (version 0.11.5)