FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100242
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA320660.7820514008685341No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG313150.7637354087880666No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA284610.6941297611214168No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA241190.5882335725549858No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG239190.5833558116813593No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG198650.48448359877295044No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG196140.4783620088765492No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC186670.4552658111399278No Hit
GTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAAT180270.4396569763443231No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT179920.43880336819143845No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT176290.42995023220580636No Hit
GAATGAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAAT164540.40129338707325074No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC156950.38278228455783825No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG144780.3531011096418211No Hit
GCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCT144520.35246700072824966No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT144340.3520280022496233No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC141800.3458332459401177No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC140920.343687031155722No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT140280.34212614767616156No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG137960.33646794506275485No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA131370.3203957229841556No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT129850.31668862472019943No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG128440.3132498033042928No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT124180.3028601726434684No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT116300.28364179480138No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC113540.2769104847957755No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG107990.263374698371462No Hit
ACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTT103760.25305823412374195No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG100090.24410754292063735No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA99620.24296126911533514No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG96990.23654701356651633No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC93170.22723049029788975No Hit
GTCTATATGCGTCTCCACATGGGGAAGATCCGAAAATCGAAACTAACAAG91760.22379166888198304No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG91320.22271856148978522No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT90510.22074306833596652No Hit
ATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG90330.22030406985734013No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA90290.22020651463986762No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG89140.21740180213753235No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC88570.2160116402885488No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA86250.2103534376751421No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC85530.20859744376063658No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC83060.20257340908170787No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA82290.20069547114536165No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG81570.19893947723085612No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT81300.19828097951291654No Hit
GAATGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAAT79700.19437877081401536No Hit
CATTTAGACAATAACTTCTCAATAAAGCAAGATAGAAAATTGCTTCTCAT77450.18889128983118555No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG76870.1874767391778339No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG76120.18564757885022395No Hit
CTACTTTTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGA75760.18476958189297119No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT73630.179574766562559No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA73370.17894065764898756No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC72690.17728221895195456No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA71990.17557500264618528No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA70540.1720386260128061No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG70530.17201423720843795No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA69850.17035579851140495No Hit
GTTGTAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCA69610.16977046720656974No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC69550.16962413438036097No Hit
GAATGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATGG69470.1694290239454159No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA68970.16820958372700928No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGACCCAA68870.16796569568332795No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC68280.16652675622560814No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT68050.1659658137251411No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC67200.16389276535384983No Hit
GAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGACTTACA65370.1594296141544816No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTT64900.15828334034917937No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC63930.15591762632547054No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC63480.15482013012890458No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG63340.15447868686775074No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG62940.15350313469302543No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA62930.1534787458886573No Hit
GTTTTATTATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT62350.15206419523530562No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTTGTTTT62290.15191786240909683No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA62200.15169836316978363No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT61280.14945459316791546No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC59770.14577188370832747No Hit
CATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG59750.1457231060995912No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC59600.14535727403406923No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG59330.14469877631612962No Hit
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT59170.14430855544623952No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG59130.14421100022876698No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT59120.14418661142439887No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTAT58920.1436988353370362No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC58610.14294278240162409No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA58370.14235745109678893No Hit
GAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGAC58270.1421135630531076No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC58170.14186967500942627No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT57750.1408453452259647No Hit
ATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGAT57670.14065023479101965No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC57440.14008929229055261No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG56640.13813818794110203No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC56080.1367724148964866No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG55880.13628463880912395No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA54860.13379698076357444No Hit
GTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTT54550.13304092782816232No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT53680.13091910184813482No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT53280.12994354967340954No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG51270.12504139999541491No Hit
CACCAGAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAA50180.12238302031928848No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG49550.12084652564409613No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA49330.12030997194799721No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT49230.12006608390431589No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG49180.11994413988247524No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATG48770.1189441989033818No Hit
CTGTAAGTCGTTTGGTTGCCTTGTTGTAATGAGGAAAATTGCATCCTACT48560.11843203401165102No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA48260.11770036988060706No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA47510.11587120955299712No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG46950.11450543650838171No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT46440.11326160748560694No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG45960.11209094487593659No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA45770.11162755759294209No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA45590.11118855911431569No Hit
GTCTAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGAT45430.11079833824442559No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCTTGTC45300.11048128378763984No Hit
GAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATG45270.11040811737453544No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATAATAAAACT45190.1102130069395904No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA45010.10977400846096401No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT44620.10882284509060684No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC44370.10821312498140354No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA44340.10813995856829912No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC43920.10711562878483757No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT43570.10626202063195295No Hit
AATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAAC43440.10594496617516722No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA43440.10594496617516722No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT43130.10518891323975511No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA42940.10472552595676062No Hit
CAGAATGAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGA42640.10399386182571661No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTT42100.10267686638983749No Hit
GAACAAGGACAAACCCTCTGGAGCAAAACAAACGATAATAAAACTTCTCC42070.10260369997673309No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCGTCCACTTTCCCT41780.10189642465005724No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA41490.10118914932338141No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA27900.0106.5758362
CAGGTAC28800.0103.24033
AGTAGAA52050.0100.205891
AGCGAAA8100.094.9305341
GGTACTG31500.094.62025
ACTGATC35100.084.8989948
GTACTGA40300.078.6373756
TACTGAT39700.075.610657
GTAGAAA72000.072.422832
GCGAAAG10900.070.52752
GTCAAAT42050.070.0227362
CGAAAGC11400.068.067033
AAATATA44600.067.1533665
TAGAAAC78550.066.749813
AGGTACT48900.065.548994
GACAGTA47300.064.534827
GACGGTA350.003566148262.147897
GGTCAAA49950.059.9790571
AGCAGGT50400.059.2995951
CAATTCG94400.055.2956929
Produced by FastQC (version 0.11.5)