FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873261
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA28700.1532087626871002No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT28610.1527283170898236No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA26700.14253219385873084No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT26400.14093070853447542No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA26110.1393826060543619No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT23550.12571659795404913No Hit
GGTGTAGGGTTTGTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCAT22800.1217128846434106No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG22270.11888359390389275No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT22240.11872344537146719No Hit
GTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCATACGTTTATTTTG21000.1121039726978782No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA20760.11082278443847386No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC20380.1087942363610837No Hit
AAGTAATATTTTCCCCCTTCTTAAATAAAAAGCATAAATCCTTAGGAATG20130.10745966525753751No Hit
GTTATAATCTCTCCTTTCCCTCACTCCCCTTTTCCCTCCTTCTGAACTCA20050.10703260250440275No Hit
ATGTATTAGTTTAGGGAAAATTAATGAAGATAAAAACAATAACAAGCACA19790.10564464855671474No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG19610.1046837573621615No Hit
GCGTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTT19490.10404316323245934No Hit
AACTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAA19240.10270859212891316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3700.064.7079851
GCGAAAG5350.044.731012
CGAAAGC6150.037.7311633
GCTATTG12700.033.1115888
GCATTAA7950.032.853451
GAGCTAT13250.032.2877856
GTAGCGG900.003616465732.2450561
CTATTGA12850.031.595789
CAATAGG16600.028.8457281
GTAGAAA11050.027.576091
GTTCGGA1606.7984E-527.2067661
TTCGGAC1100.00966171126.3703462
ATAGGAG19800.025.6364673
TAGTACT14450.025.5920144
AATAGGA20350.024.5885662
TTAGAGC18150.023.9717623
GTCTAGT7150.023.3382051
TAAGTCG1902.1939518E-422.895069
ACCGTGC10400.022.3086038
CTTAGTG9250.021.9462579
Produced by FastQC (version 0.11.5)