FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627073
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG144430.3121411743449909No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA137550.29727216320122896No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA109490.23662907414687429No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC97310.21030573755806314No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG95580.2065668728373207No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG87200.18845607147326182No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC80470.17391123935152958No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG74670.16137631716638143No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG68580.14821464887197586No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC65810.14222814293182753No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA63180.1365442040789069No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT61700.13334563772821392No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT61400.13269727968415454No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG56930.12303674482766969No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT52270.1129655832099472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA15000.074.957931
GCGAAAG21000.053.5228652
GGTACTG29800.052.5576935
GCAGGTA29450.052.2008482
CGAAAGC21850.051.1072043
GTACTGA44050.051.0287066
CAATTCG46100.050.1675459
CAGGTAC30550.049.3702133
ACTGATC34200.045.7904368
GTAGAAA55250.043.3270231
AAGGTCG31400.042.252719
TACTGAT39650.038.7654237
AGTAGAA56450.037.265871
CAGTATG43400.036.2495889
GGTCGTT26150.036.0432177
TATATTC65200.035.255313
GGTCAAA43500.035.1860431
AGGTCGT28100.034.0617076
GACAGTA50250.033.3294227
CTAGTAC25150.032.8684923
Produced by FastQC (version 0.11.5)