FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271784
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11950.054.025441
CAATTCG11300.046.194079
CGAAAGC14450.044.6638343
GCGAAAG15550.042.4390032
GACCGTC3950.031.204677
AGTACTG31000.026.9007825
TAGTACT41950.026.447724
CTAGTAC15200.026.2393423
GGTCGTT8800.025.541527
CCTTATA20500.025.1164722
GTAGAAA35150.024.5582751
GTATTAG17800.024.0440851
TGTATCG1550.001807255823.3919835
TATACTG38200.023.3491175
GTATACC14150.023.0613544
ACCGTCA4455.456968E-1222.809758
TATATAC11900.022.546993
TATCGGT2303.1434658E-522.0667297
GTATCGG2003.1009552E-421.7545456
TATTAGG14150.021.525192
Produced by FastQC (version 0.11.5)