FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872436
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCG848952.192289298002601No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAA507201.3097698709546135No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA498361.2869418629513825No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT458611.1842932975522384No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT337190.8707438935078591No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGA321620.8305366441175528No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT280940.7254864896411458No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC279300.7212514293328541No Hit
GGATAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA275930.7125488968700838No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA225650.582708145467091No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG218730.5648382568491771No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG207940.5369746588452333No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG197430.5098341199183151No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG191130.49356529068524313No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA190810.4927389374543569No Hit
GCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGAT180890.467121987296885No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA164090.42373844267535987No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG161770.417747381751435No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG151100.39019366620907353No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA150850.38954807774744377No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGA147820.3817235455924901No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT135680.3503737698957452No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA129550.3345439408165816No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG121500.3137559923521009No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA118210.3052600481970522No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC117250.3027809885043936No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG115000.29697069234972506No Hit
ATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCT112030.28930110142556265No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA105480.2723866837308609No Hit
TGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGA103050.2661115638838189No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG102450.26456215157590723No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC102390.2644072103451161No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT101630.2624446214217614No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT101380.2617990329601316No Hit
ATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG99580.25715079603639673No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT99060.2558079720362067No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT97390.25149544111251937No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACA97260.2511597351124718No Hit
CCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAG95800.24738949849655356No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG92520.23891937787997014No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA91490.2362595534180552No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG83700.21614301695366947No Hit
GCATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA83260.21500678126120096No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA80540.20798277879866836No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGC78800.20348948310572468No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT78530.20279224756716446No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG78310.2022241297209302No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT77720.20070054095148374No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC76230.19685283372016996No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG75740.19558748033537543No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA73040.1886151249497732No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT71040.18345041725673453No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA70320.1815911224872406No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT68970.17810494479443947No Hit
CCATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAAT68620.1772011209481577No Hit
CCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATTCGA66760.17239794279363171No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT64810.167362352792919No Hit
AAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG64710.16710411740826706No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC64430.16638105833124164No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC63320.16351464556160514No Hit
TCATATAGGAGACCGGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCG63210.16323058663848802No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG61810.15961529125336094No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA61540.15891805571480072No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG61340.15840158494549683No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA61180.15798840833005373No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC61060.15767852586847142No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC60560.15638734894521175No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC58220.15034464094435646No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG58220.15034464094435646No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG57340.14807216955941943No Hit
GGCCTCCACCATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGG56960.1470908750977421No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT56400.14564475694369125No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA54710.14128057894307355No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA53420.1379493424810636No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT53070.13704551863478182No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG52120.13459228248058844No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT51420.1327846347880249No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT51390.13270716417262932No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATT51350.13260387001876855No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT50680.1308736929416006No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT50330.1299698690953188No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG49980.12906604524903703No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT49860.12875616278745472No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA49730.12842045678740718No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC48640.12560569109470113No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA48560.12539910278697958No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG47520.12271345478659945No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC46840.12095745417096629No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG46680.12054427755552319No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA45590.11772951186281709No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA45540.11760039417049115No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT45480.11744545293969996No Hit
GGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGG45340.11708392340118727No Hit
GCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCG45320.11703227632425686No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC45030.11628339370876627No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG44590.11514715801629775No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA44580.11512133447783256No Hit
CAAACAGACTGTGTTCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGT42900.11078298001568004No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG42580.10995662678479387No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG42320.10928521478469884No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT42180.10892368524618612No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA42080.10866544986153417No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC41880.10814897909223033No Hit
GAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCC40710.10512762509180269No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT39820.10282933016840046No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG39760.1026743889376093No Hit
GCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTG39660.10241615355295736No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG39350.10161562386053635No Hit
AAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAAT38750.10006621155262474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA73000.0116.05591
AGCGAAA8150.0102.3499151
GTAGAAA95300.089.24852
GCAGGTA9200.084.331872
TAGAAAC102450.083.301863
GAGCGAA1700.076.80171
GCGAAAG11050.075.4625242
CAGGTAC10550.073.5396353
CGAAAGC11450.072.825343
GGTCAAA36700.071.744361
ACTGATC11450.069.0195168
AGAAACA124500.068.896954
GAAACAA131650.064.879725
ATACCGG1250.063.830741
GCAGGCA25600.063.44434
GTCAAAT41350.063.3034632
GGTACTG12850.062.068915
GACAGTA20400.059.3522537
TAATCCG753.76127E-758.0062185
GTACTGA16000.056.6466946
Produced by FastQC (version 0.11.5)