FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627773
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA43460.1653871928815769No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA42480.16165779920868356No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT33170.12622855931619664No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG32910.12523912834175555No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC31090.11831311152066788No Hit
GCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGTTTTGT28520.10853296688869245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6500.051.33821
GCGAAAG10050.034.6474531
CGTCGTG1707.8307494E-834.117794145
AGCTATC16700.033.861789
CGAAAGC9950.033.5279432
CTATTGA20050.032.543169
GCTATTG20800.032.0680588
GTAGAAA18950.031.007871
GGCGATC950.004728140330.5270298
GCATTAA14150.029.7349821
TAGAGCT24400.029.4205914
CAATAGG25650.029.130371
GTATACG1504.3955595E-529.0056463
GTATACC8000.029.0045414
ATAGGAG27700.028.7962193
GAGCTAT25750.027.5965526
TTAGAGC26400.027.1927933
GGAGCTA22500.026.1011077
AATAGGA30400.025.7645342
TAGGAGA34750.025.4572264
Produced by FastQC (version 0.11.5)