FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425589
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC66290.27329444518424184No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG53910.22225529551791337No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50800.20943366745149322No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTTCTCACATA36940.15229290700114487No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA33560.13835814723764No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT31780.13101972345685933No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC30490.12570142757078795No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT30180.12442338747413516No Hit
GGTGTAGGGTTTGTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCAT26030.10731414101894426No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC25690.10591241962261537No Hit
GTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCATACGTTTATTTTG25270.10418088142715026No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT25270.10418088142715026No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA25080.10339756652920178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC18450.057.763698
GCAGGTA19400.053.073082
CAGGTAC20050.052.0747073
GGTACTG21900.047.6756975
GTACTGA24050.046.4284636
GACAGTA20700.043.430447
TACTGAT27500.039.54597
GCTATTG11900.037.7727558
CGAAAGC5800.037.5042882
CAGTATG24650.036.4702459
GGATAGC24250.035.879655
AGCGAAA4550.035.072691
CTATCGA850.002735955234.134921
GCGAAAG6450.033.7381
ACAGTAT31900.033.408788
ATCTATC6850.032.809918
CTGATCC34350.032.5033349
AGGTACT36450.031.2306354
TAGAGCT15950.030.45744
GCGCCTT2951.4551915E-1129.49125145
Produced by FastQC (version 0.11.5)