FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5440165
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT93090.17111613342610013No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC77900.14319418620574928No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG67140.12341537434985886No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA66550.12233084842095783No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA58620.10775408466471145No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG55120.10132045627292555No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC55000.10109987472806431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11300.046.2186661
GCATTAA41400.041.8755871
CATTAAG46750.037.384792
CGAAAGC14850.035.1597373
CGTACCG1051.8524495E-434.5312465
CCCTTAT28600.033.732531
GCGAAAG16800.031.0801222
GTCTAGT24100.031.0015581
GCTATTG37300.026.629778
TTATACT47400.025.3957654
TAGAGCT44850.025.3845084
TAGTACC26000.024.8226554
CTATTGA41700.024.5140349
TTAGTAC39450.024.4480483
GTATACC18250.023.8407524
TATACCC22700.023.6394525
TAAGATG82850.023.54465
ATAGTAC48850.023.4548193
GTAGAAA41200.023.4106752
ACCGTGC37500.023.2009458
Produced by FastQC (version 0.11.5)