FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836721
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC116160.19901585153719015No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT112120.1920941569761515No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC67510.11566425738012832No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC66670.11422509316446683No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT66360.11369397303725842No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA63930.10953067655623766No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC62540.10714920243746445No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA61230.1049047915773257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13750.059.6097951
GCGAAAG17450.048.203012
CGAAAGC18600.044.8313333
TATACTG42200.039.0034185
TTAGGTA28250.036.703454
GCTATAT11400.029.9043871
CAATTCG4050.028.6416789
ATTAGGT39650.028.3455733
TAGTACT24950.027.3178564
TAGGTAG43550.027.138685
ATACTGT50100.026.3404376
CTAGTAC19050.026.2633933
GTAATAT43650.025.091961
GATTAGG42500.024.7394872
GTATTGA7200.024.1780151
GGATTAG45650.023.6748561
ATATACT57150.023.0910914
AATATAC52650.022.310713
AGGTAGG47900.022.100736
GTAGAAA31250.021.8116972
Produced by FastQC (version 0.11.5)