FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524981
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG90420.25651202091585745No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC83480.2368239715334636No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA78420.22246928423160292No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT78030.22136289528936468No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA72230.20490890589197502No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG71340.20238406958789282No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC71300.2022705937989453No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC70730.20065356380644322No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC68030.19299394805248596No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT66710.18924924701721796No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT66020.18729178965787333No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA64410.18272438915273587No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG63290.17954706706220544No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA63060.17889458127575722No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT62600.1775896097028608No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT62550.1774477649666764No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT62420.177078968652597No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC61030.17313568498667087No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT59540.16890871184837591No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC59110.16768884711719012No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT58620.1662987687025831No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT58100.16482358344626538No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT57640.16351861187336897No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC57520.1631781845065264No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT57400.16283775713968388No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT56140.15926326978783714No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA55860.15846893926520456No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT55140.15642637506414928No Hit
GTCTAGAATTGACCTGCATGGTACCCGGGCATGCTCGAGCTAGCAGATCT53170.15083769245848416No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA51970.14743341879005872No Hit
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGA51730.14675256405637363No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC50550.14340502828242194No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT50240.1425255909180787No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG50100.1421284256567624No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT49820.14133409513412978No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA49750.14113551250347164No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG49400.1401425993501809No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA49010.13903621040794262No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA48940.13883762777728445No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG48430.13739081146820364No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA47790.13557519884504343No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT47380.1344120720083314No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG46970.13324894517161937No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG46740.13259645938517114No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT46250.13120638097056408No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT46110.1308092157092478No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG46100.13078084676201093No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT45790.12990140939766767No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT45720.12970282676700953No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC45640.1294758751891145No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC45530.12916381676950883No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT45350.128653175719245No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC45130.12802905888003369No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC45080.12788721414384926No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG44310.12570280520660962No Hit
CCCCAGGACACACGCGGGAGCAGCGCCGGGCCGGGGACGCCCTCCCGGCC43820.12431272679200257No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA43790.12422761995029193No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA43110.12229853153818418No Hit
GTACCCGGGCATGCTCGAGCTAGCAGATCTTTTTCCCTCTGCCAAAAATT42900.12170278364620972No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA42250.1198588020758126No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC41900.11886588892252187No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA41340.11727722787725665No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC41170.1167949557742297No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG40930.1161141010405446No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC40880.11597225630436021No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA40300.11432685736462125No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG39910.11322046842238298No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG39740.11273819631935605No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC39450.11191549684948657No Hit
GCATATGGGCATATGTTGCCAAACTCTAAACCAAATACTCATTCTGATGT39250.111348117904749No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG39170.11112116632685397No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT39150.1110644284323802No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT38750.10992967054290505No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT38300.10865306791724551No Hit
GTGTGTGGCTGCGATGGTGGCGTTTTTGGGGACAGGTGTCCGTGTCGCGC37990.10777363055290227No Hit
GCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG37450.10624170740211082No Hit
GCCACACACGGAGCGCCCGGGGCCCTCTGGTCAACCCCAGGACACACGCG37430.10618496950763708No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT37360.10598638687697891No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT37100.10524879424882007No Hit
ATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGG36780.10434098793723995No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA36390.10323459899500168No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC36390.10323459899500168No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG36150.1025537442613166No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT36140.1025253753140797No Hit
CTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACTGACAATAACT36060.10229842373618468No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA35970.10204310321105275No Hit
CTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTG35750.10141898637184145No Hit
GTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATA35640.10110692795223578No Hit
GTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCA35580.10093671426881448No Hit
ATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGCG35350.10028422848236627No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT35320.10019912164065566No Hit
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG35280.10008564585170814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTG450.0128.88747145
AATCGCG353.438671E-582.85623145
GCTTGCG651.5661499E-978.07607145
TTTCGCG2150.077.5573145
GAGATGG1850.074.494571
GCCCTGG1250.069.59924145
CAGGGCG3700.066.633184
AGATGGG2100.065.611272
TTAGGGG5950.062.153644
TAGGCAT350.003564800662.1536335
GTCGGCC1051.6370905E-1162.142178145
TAGGGGC5950.060.934945
GGACGGG1007.166818E-1058.027351
AGGGCGG4250.058.010065
GCACACC3550.055.14024145
GGGCAGG4400.054.400641
GAGTGGT400.00604799754.384434
TCACCGC3250.053.537872145
AGAAATA1650.052.726692145
AGGGGCG6900.052.5453456
Produced by FastQC (version 0.11.5)