FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620170
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA725332.76825549487247No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT373511.4255181915677226No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA345441.3183877382001932No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT243700.9300923222538995No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC223920.854601037337272No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA219190.8365487735528612No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG206020.7862848593793532No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG198640.7581187480201667No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA178170.6799940461878428No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA177660.6780476075979803No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG164690.628547002675399No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG147820.5641618673597515No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG139630.5329043535343126No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG134390.5129056511600392No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG128540.4905788555704401No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG118680.45294770949976526No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA113510.4332161653633161No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA113020.4313460576985463No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA105300.40188232061278467No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG104950.40054652942366337No Hit
GGATACACCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGG91590.3495574714617754No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA91010.3473438746340886No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT90950.3471148818588107No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG90750.34635157260788424No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC88750.33871848009861955No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC85070.3246735898815726No Hit
CCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGGAATCGTT83510.3187197777243461No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA80270.3063541678593374No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT80020.3054000312956793No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC77530.2958968311216448No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG77340.29517168733326465No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC77020.2939503925317823No Hit
ACTTAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAA76440.29173679570409555No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG76430.29169863024154924No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA75970.2899430189644183No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT73750.28147028627913456No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC73600.2808978043409397No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT72900.2782262219626971No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA72000.274791330333528No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA71360.2723487407305633No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT70840.2703641366781545No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC69640.26578428117259567No Hit
CTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC68580.2617387421426854No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG67720.2584565123637016No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT67290.25681539747420973No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG65880.2514340672551781No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACATCTG65320.249296801352584No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG64260.24525126232267372No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA63830.24361014743318182No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA62840.23983176664109582No Hit
GGATACACCATGGATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGA61530.23483209104752747No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA61340.23410694725914732No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG61090.2331528106954892No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA60610.23132086849326572No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT58900.22479457439784442No Hit
ACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATG56190.21445173404779078No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT55040.2100627058549636No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT53920.2057881740497754No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATAGGAATCGTTCTA53740.2051011957239416No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAGCACCACATTC53580.2044905483232004No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG52900.2018952968700504No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC51160.19525450638699016No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA50820.19395688066041517No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTT50070.1910944709694409No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG48760.18609479537587256No Hit
CCTAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAA48210.1839956949358248No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC47840.1825835728216108No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC47770.18231641458378653No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT46470.17735490445276453No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA46000.17556112771308732No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT44060.1681570279791006No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA43870.16743188419072044No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC43540.16617242392669176No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAA43290.1652182873630337No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG42960.16395882709900503No Hit
TCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG42560.1624322085971521No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA42520.1622795467469668No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT42240.16121091379566976No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT41970.16018044630691902No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG41840.1596842952938168No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC41460.15823400771705654No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA41220.15731803661594476No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTGCGCAAAATGCCA40390.15415030322459994No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT40300.153806814061683No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT39950.1524710228725617No Hit
GACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGA39750.15170771362163524No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT39740.15166954815908892No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA39610.1511733971459867No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT39410.15041008789506025No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC38760.14792933282954923No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA38480.14686069987825218No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA38220.14586839785204778No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATT38190.14575390146440878No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC38030.14514325406366763No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA37760.14411278657491688No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC37430.1428533263108882No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT36430.1390367800562559No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC35330.13483857917616032No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG35100.1339607735375949No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC34940.13335012613685374No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATTCCTGGA34550.1318616730975471No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA34370.13117469477171328No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC34350.13109836384662066No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA34230.13064037829606476No Hit
GTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATG34210.1305640473709721No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG33850.12919009071930448No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT33610.1282741196181927No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG33440.12762530675490522No Hit
GAATGACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGT33000.12594602640286698No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC32810.12522088261448686No Hit
GTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCA32440.12380876050027288No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG32310.12331260948717068No Hit
GAACTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCC31940.12190048737295671No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA31890.1217096600602251No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC31680.12090818534675231No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC31460.12006854517073319No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA31370.11972505600781629No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT30960.11816027204341703No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA30180.11518336596480382No Hit
ATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG30160.11510703503971116No Hit
GACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCC30080.11480171133934058No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC30060.11472538041424794No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA30010.1145345531015163No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC29920.11419106393859942No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA29390.11216829442364426No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC29310.11186297072327368No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG29240.11159581248544942No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG29170.11132865424762517No Hit
GCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTTTTCCCTA29110.11109966147234722No Hit
ATTCCCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT28710.10957304297049429No Hit
GAACAGGATACACCATGGACACAGTAAACAGACACATTCCTGGATTCCCA28250.10781743169336341No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA28120.1073212806802612No Hit
CTTCAATGGTGGAACAGATCTTATGATCTCAGAGAACTCTTCTTTCTTGA27860.1063289786540568No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA27850.10629081319151047No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA27790.10606182041623255No Hit
GAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTG27650.10552750394058402No Hit
ATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCG27590.10529851116530607No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG27470.1048405256147502No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC27430.10468786376456489No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGG27400.10457336737692592No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT27280.10411538182637005No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT27050.1032375761878046No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA27020.10312307980016565No Hit
GAACAGGAACAGGATACACCATGGATTCGAGAAATTTTTCCCTAGCAGTT26550.10132930306048844No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA26450.1009476484350252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT100.0070764506145.103161
AGTAGAA60100.0112.147121
GAGCGAA1000.0108.827371
CAACGGT203.8640478E-4108.769214
AGCGAAA6150.0106.173041
GCAGGTA13600.0105.046962
CCGCACG1150.0100.86813145
CAGGTAC14750.096.8531653
TTCGCCG451.0910298E-896.683754
GGTCAAA21600.094.720121
GTAGAAA78900.085.571442
GCAGGCA17350.085.260014
AGCAGGC17550.084.293213
TAGAAAC80500.083.867443
GGTACTG17250.083.2320865
GTCAAAT24350.083.0924152
AGAAACA82850.081.746484
ACTGATC17500.081.200398
TACTGAT17600.080.3316967
AGCAGGT18850.076.208031
Produced by FastQC (version 0.11.5)