FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082965
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC102830.3335425475151356No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT101990.3308178977056178No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC77820.25241934306746916No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG66440.2155068254099544No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT61650.1999698342342518No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT59050.1915363943476491No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC56710.1839462984497067No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC54310.1761615847082273No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA53080.17217191891571912No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG52480.17022574048034927No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA45450.14742301647926592No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG45000.1459633826527385No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA43270.14035190149742213No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA42580.1381137962967468No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG41600.1349350381856427No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA39770.12899919395776469No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT39760.12896675765050852No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA38700.12552850908135513No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT35350.11466234615054015No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT34500.11190526003376619No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA32980.10697494133082926No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC32050.10395836475600598No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG31670.10272578508027175No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT31000.10055255249410876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAA32850.054.1014561
AGCGAAA7800.053.9400831
AGATGTA39600.044.674697
GCGAAAG10000.042.7889442
CATTAAG46150.040.072652
TAAGATG47000.039.8056835
TTAAGAT46100.038.2233354
CGAAAGC11250.038.0315363
AAGATGT53000.035.5730176
GTAGAAA28450.034.9314421
CTATTGA27250.033.7884949
GAGCTAT27650.033.3067136
GCTATTG31050.029.653838
TTAGAGC37700.027.3143793
GTGTACG14150.027.1550799
TAGACGG1100.00966540226.3688095
AGAGCTA35150.026.2000225
ATAGGAG23150.025.6866343
CAATAGG23700.024.4861151
ACCGTGC38750.023.7613268
Produced by FastQC (version 0.11.5)