FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072674
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC27040.13045949338873358No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT23780.11473101896390846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5300.053.380431
CGAAAGC6400.045.3238333
GCGAAAG6600.042.854732
GTAGAAA22000.035.9320372
AGTAGAA23350.033.5529141
GAGCGAA3251.8189894E-1229.0170571
AGTACTG16300.028.9168765
CCCTTAT11700.027.9010161
TAGTACT20200.026.9237524
CCTTATA14550.026.4174542
TACTTCG1100.00967351726.3638697
TATATAC8350.025.1859383
TTATACT17200.024.4526084
TAGAAAC33100.024.3187683
CAGTCCG8500.023.029069
GGACAGT24850.022.7611316
ACAAGGC17700.022.5279058
AGGCCGT3554.12183E-922.4693246
TATACTG18200.022.3121955
TATACAG4307.094059E-1121.9230275
Produced by FastQC (version 0.11.5)