FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701612
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC63320.17106060818908086No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC39270.10608891477550861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7950.057.4800531
GCGAAAG9300.049.9066772
CGAAAGC10850.044.1121443
GTAGAAA26900.033.1662641
AGTAGAA26250.032.605921
GGTCGTT1652.3872053E-630.7600947
AGTACTG17300.027.6645875
TAGTACT23950.026.9470274
GACCGTC3255.0931703E-1126.7714237
GTATAGG9650.026.3077951
CTATACG5250.024.868921
GCATTAA24800.024.5680891
GTATACC7700.023.5437534
ATATAGT17900.023.5027521
CTAGTCG1550.001809541523.38689
CCCTTAT14150.022.042241
GGATAGC4951.8189894E-1221.9741695
GAGCTAT14300.021.8051386
GTCTAGT13450.021.5715541
CATTAAG26950.021.527462
Produced by FastQC (version 0.11.5)