FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763787
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA204850.7411931527284845No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG195200.7062772927146701No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA157440.5696531606813404No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT154220.5580024799306169No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA148430.5370529639223284No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC146920.5315894459305294No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT136240.4929468153660177No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG134050.48502290516599145No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG131770.47677335482075855No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG109660.396774425815014No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG108140.39127472558485876No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC107740.3898274360506074No Hit
GTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAAT106130.38400209567524557No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT100120.3622565704231187No Hit
GAATGAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAAT98270.35556285632720613No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT94670.34253725051894374No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG88150.3189464311106464No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT87180.3154367539900868No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC86920.3144960157928234No Hit
GCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCT84830.30693392797636No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC83560.30233878370511186No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC83210.3010724053626419No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT81440.2946681491735796No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG79990.2894217246119184No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT79560.28786588836259813No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA77600.2807741696447664No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG71380.25826881738715757No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG67820.24538794053232033No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG66400.24025006268572793No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG64070.2318196011487137No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG61650.2230634994664929No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT61320.2218694856007355No Hit
ACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTT60410.21857690191031365No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA59390.2148863135979726No Hit
CATTTAGACAATAACTTCTCAATAAAGCAAGATAGAAAATTGCTTCTCAT59280.2144883089760535No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC58980.21340284182536495No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA58780.2126791970582393No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC58250.21076153842535622No Hit
ATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG57640.20855442188562287No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG57030.20634730534588955No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG56420.20414018880615617No Hit
GTCTATATGCGTCTCCACATGGGGAAGATCCGAAAATCGAAACTAACAAG55340.20023250706367748No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA54490.19715701680339334No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC53400.19321315282255833No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC52290.18919692436501076No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC51920.18785818154582823No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG51510.18637470977322057No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG51490.18630234529650802No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG50730.1835524951814304No Hit
CTACTTTTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGA48900.17693114556223039No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC48550.17566476721976043No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA48540.17562858498140413No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA48520.17555622050469155No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC48500.175483856027979No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA47430.17161235652385656No Hit
GAATGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAAT47210.17081634728001832No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT46090.16676393658411448No Hit
GTTGTAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCA45910.16611265629370137No Hit
CATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG45730.16546137600328825No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA45720.16542519376493195No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC44960.16267534364985436No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG44540.1611556896388904No Hit
GTTTTATTATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT44140.15970840010463902No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTTGTTTT44060.15941894219778874No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT44030.1593103954827199No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG44000.15920184876765103No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT43960.1590571198142259No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT43950.15902093757586963No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA43930.15894857309915705No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG43910.1588762086224445No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC43730.1582249283320314No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA42960.15543889597859747No Hit
GAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGACTTACA42830.1549685268799658No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA42160.15254431691009473No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT42060.15218249452653188No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA41790.15120557409091223No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG41300.1494326444114543No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTT41160.1489260930744663No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGACCCAA40170.14534405147719415No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA40120.14516314028541275No Hit
GTCTAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGAT39730.14375203298951764No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC39110.141508734211428No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTAT38770.14027853810731433No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC38390.13890361304977553No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG38270.13846942618950012No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC38230.138324697236075No Hit
GAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGAC38140.13799905709086843No Hit
GTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTT37230.13470647340044656No Hit
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT36830.1332591838661952No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC36560.1322822634305755No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA36150.13079879165796784No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA36070.13050933375111756No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT36000.1302560580826236No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC35610.1288449507867285No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT35370.12797657706617768No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC35330.12783184811275256No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC35270.12761475468261485No Hit
ATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGAT35020.12671019872370773No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG34650.1253714559045252No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT34400.1244668999456181No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC34210.1237794374168487No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG33980.12294724593465416No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT33910.12269397026616016No Hit
CACCAGAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAA33840.12244069459766617No Hit
GAATGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATGG33620.12164468535382793No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG33450.12102958730177107No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT33190.1200888491045077No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATG33000.11940138657573829No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA32710.11835210166340605No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT32550.11777318584970549No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA32050.11596407393189127No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA31990.11574698050175357No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA31860.11527661140312187No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCTTGTC31720.1147700600661339No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT31400.1136122284387328No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC30860.11165838756749344No Hit
CTGTAAGTCGTTTGGTTGCCTTGTTGTAATGAGGAAAATTGCATCCTACT30630.11082619608529891No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA30570.1106091026551612No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCGTCCACTTTCCCT30510.1103920092250235No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT30070.10879999073734699No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT29870.1080763459702213No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA29510.10677378538939505No Hit
GAATCAAATCGGAAACATGGCCAATCGGAGAGTCACCCAGGGGAGTGGAG29500.10673760315103877No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT29200.10565213600035024No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT29170.1055435892852814No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA29000.10492849123322455No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA28730.10395157079760489No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG28640.10362593065239832No Hit
GAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATG28580.10340883722226062No Hit
AATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAAC28560.10333647274554805No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA28400.10275755693184749No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA28340.10254046350170978No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA28240.10217864111814695No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA27840.10073135158389557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5050.0109.159131
GCAGGTA22050.0104.577552
CAGGTAC22850.0100.9125443
AGTAGAA39900.099.801551
GGTACTG26650.086.521955
ACTGATC27100.085.345088
GTACTGA28300.083.527126
TACTGAT31650.072.617727
TCGGACA300.001935073272.509744
GCGAAAG7850.072.051812
GTAGAAA55800.071.7339552
AGCAGGT33450.069.6061
TAGAAAC59100.067.8487553
AGGTACT35700.067.025814
CGAAAGC8300.066.395663
GACAGTA32550.064.818517
AAATATA25750.064.20285
GTCAAAT27500.060.1204342
GGTCAAA28950.058.6280061
AGAAACA69300.057.8613134
Produced by FastQC (version 0.11.5)