FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_E04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_E04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGAGAGCCTGAAATCCAAACCAGGGCTCCCAGAAAACCACCAACA22.8169014084507045No Hit
GGCCTCATGGTCTGAAGTACGTACCACGCACTGCCATTAAATCTCAAGCT22.8169014084507045No Hit
ATCCTGGAGTTCTTCTTTCTTCTCTTTGGTTATCGTTAGTTGCGATTCCG11.4084507042253522No Hit
TATACAAATTAAGGGGCAGATTATGCAGAAATTTTTAGGAAAAGCGTGGC11.4084507042253522No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.4084507042253522No Hit
ATAGATATCATTGTCAATATTAAACAAAAATTTGCCAAAAGTTTAATATT11.4084507042253522No Hit
CCCGAAGACTTGAGAGAATGCCAAAAACCATCTAACTTGCAAAGGAAAAA11.4084507042253522No Hit
GGTGATGGTGATGCAGCCGCGCTCGTCCTCGATGACCCGGCGCGCGATAG11.4084507042253522No Hit
GTTAAAGTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGCACAGATC11.4084507042253522No Hit
CTGTGTGGCTGTTCACAGGCCTGTTCGGAGGACAGGAACGGCGTCGTCAC11.4084507042253522No Hit
GTAAAATGGAGACAGTCGTAATACCTGTACCTCAGGCAGGCAGAGAAGAT11.4084507042253522No Hit
GTTTCAAGAGTAATATTTTTATTTGATTTTAATATCATCAACCACTGCTG11.4084507042253522No Hit
GGCCCACTGCAGGGATAATTAATACATAATGAAGGGAGTGTACATAAATA11.4084507042253522No Hit
CGATAAATACATTTTAAAAAAAGACGATCAGTAATACACAAAATCAGCTG11.4084507042253522No Hit
GATAAATTTGAGGGTGTTCTGGAGTGGCATCGAATGCCTTTTAAACCGTG11.4084507042253522No Hit
GTTTTTATCTTCTTTAATTTTCCCTAAACAAATACATTGGCTCGTTGTAA11.4084507042253522No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGCCCAGCAAT11.4084507042253522No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGTTT11.4084507042253522No Hit
CCTTTATCGTTGCTGCCCCAACATCAGCACCCCTGTCCTCTTGCTAATTT11.4084507042253522No Hit
TCCCCTACTCTCATCCAGACATTTTACTTGGAGGGGTTCTGTGTAAGAAA11.4084507042253522No Hit
GTGTTGTCCACGCTACCCTGCTTTGTCTGTTTCGAGCATAAGTGGGTGTG11.4084507042253522No Hit
ACTTAATTATCAGATGGTCTAGGAGAGCCTGAAATCCAAACCAGGGCTCC11.4084507042253522No Hit
GTGCCAACAGGGCCGAGGAATGAAATGCTGGTCACACAACCACTCTACTA11.4084507042253522No Hit
GTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTT11.4084507042253522No Hit
TGGAAATTCTCAGTAGGTTTCGGACCACCTAGTTGTTCTTGATTTGGCGC11.4084507042253522No Hit
GGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAACATTGTG11.4084507042253522No Hit
GAGACCTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGCCCCTGGCATC11.4084507042253522No Hit
TTTATGAAGAAGACATCCACTCCCAATAATATCCCGCCTAGACCTCACCG11.4084507042253522No Hit
ATAAAAAGCCCAGCACATCCATATGCATATTCTCTGTGTCAAATTTATTA11.4084507042253522No Hit
GTTGTATAGTTTACTATTTAATTTTTAATTTTTTCTACTTCTGTCTCTTA11.4084507042253522No Hit
GTTATGGGTGGTCTCCTCTGCAGTAGTGGGTAAGGGGACTAGACTGAGAT11.4084507042253522No Hit
ATCAAAGACTACAGATACACATATAGGTGCCATAGGGGAGACACACAAAT11.4084507042253522No Hit
TCCCCGGAGCGAATCGACCCCGGCAACCCGACGTACACGTTCGTCAATAC11.4084507042253522No Hit
TTTTTTGGATATTGATACCCATTTTCTGGTTTCCTATGTTTCCAGCAACC11.4084507042253522No Hit
GTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCCCAAT11.4084507042253522No Hit
GTTCATGCCCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT11.4084507042253522No Hit
TTATTCCACAGATCACTAAAGTTATATTTAATTTTTTTTTTTAAGCTTTT11.4084507042253522No Hit
GTCTATTTTGTTTTACAACCTGCTTTTTCCCCTCTACTATTTGTTAAATG11.4084507042253522No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATAGATGCTAAATGC11.4084507042253522No Hit
TAGTAGTACTACTACTAGTAGTAGTAGTACTACTACTACTACTAGTAGTA11.4084507042253522No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC11.4084507042253522No Hit
ATTCCCAGCTGACACTTCTGACTGCTGTGTGGGGAGAGCCTCGGAGAAGG11.4084507042253522No Hit
CCCCTTTTCCCTCCTTCTGAACTCAGTTTGGTGTGTGTCTTTTCTTCACG11.4084507042253522No Hit
GAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAAGCACAGCAGC11.4084507042253522No Hit
TGTTAATACAGCGCTGTATTAACATGTCAGCGCTGACATGTTAATACAGC11.4084507042253522No Hit
TACGAGAACTTAAGCATATGCTTTTTTAGCTTATCCTGAGATTTCTGTTG11.4084507042253522No Hit
CTTTACAAACAGAGTGTTTCCAAACTGCTGAATGAAAAGAAAAGTTAAAC11.4084507042253522No Hit
GGCGTAGCTGACTAAGACATCACGTCGAGATTTTTAGTGATTATGGAAAG11.4084507042253522No Hit
CTCCTGATTTGACCTCAGAGTCAAGCTTGTCCAAATCAATCATTTCACAT11.4084507042253522No Hit
AGTAGAAACAAGCACATTGCCAAGTAGAGTGTAAAAGTGAGTTATAAAAT11.4084507042253522No Hit
CAAAGACCACTTTGGAGCATATGGCCATAATAAAAAAGTACACATCATTA11.4084507042253522No Hit
GTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGT11.4084507042253522No Hit
CCCATATAGGAATCAGAATCTTATCAACATTACAGTCCTTTCCAACAAAA11.4084507042253522No Hit
AATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCT11.4084507042253522No Hit
AAACAAGGGGTTTACTTAATTATCAGATGGTCTAGGAGAGCCTGAAATCC11.4084507042253522No Hit
GGGTGTGACATCACTGGAACAGGGGTGGTTCCAGTCAGGGAGTGGGGAGA11.4084507042253522No Hit
CTTTAATACAATAAAAAAAAAAAAAATTGTGGGGGACGGTGAGGCAATCT11.4084507042253522No Hit
AATTAGCACATTAGCAAGATTGCTCAGTTCATTGATGCTTAATGCATTCT11.4084507042253522No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA11.4084507042253522No Hit
CCATTTTACAGATGAATGGACTGAGACTGGAGGCGACATGAAGCTCTGGG11.4084507042253522No Hit
AACAGATAAAATAAAATAACAAAAAACACACACCAAAATCATTCAAAATT11.4084507042253522No Hit
CCCTTTGACAATAAGAAAAATATACTTGGAGAATTATAAGATCATGATAA11.4084507042253522No Hit
CAGTGCAATCCTGCCAGCAAAAGGGGGACAAGGGGATGTGGGGGTAAAAG11.4084507042253522No Hit
TATTAGTTTAGGGAAAATTAATGAAGATAAAAACAATAACAAGCACATGA11.4084507042253522No Hit
CCTTATGTCTTGGGCTTCACGCATGCTACAATGGCCGGTACAATGGCTGT11.4084507042253522No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC11.4084507042253522No Hit
CTACTTCTTCCGCCCGCCTCTCTCCTTGAGGGCCAGGTGGATGATGGCGA11.4084507042253522No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTACCCGTT11.4084507042253522No Hit
GTCCAGTTGAGCTTTGTTTTGCTCAAATGTCGGATCAGTCATTTATTTTT11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAAAA50.0145.0145
GAGCAAA50.0145.01
AAAAGAG50.0145.05
GCAAAAG50.0145.03
AGCAAAA50.0145.02
CAAAAGA50.0145.04
AAGAGAG100.072.57
GAGAGAG100.072.59
AAAGAGA100.072.56
AGAGAGA150.048.3333328
Produced by FastQC (version 0.11.5)