FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433315
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA74040.16700820943244502No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA69920.1577149379189162No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT69500.15676756558015842No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG60380.13619605193856066No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG55140.12437645418834438No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT55080.12424111528280757No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG52460.11833131640769942No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG51060.11517340861184013No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC51010.11506062619055944No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA47930.10811322903966897No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC47750.10770721232305847No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA45600.10285756820798883No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC45550.10274478578670813No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG44580.10055680681386277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9350.067.493281
CGAAAGC13350.047.2567143
CAATTCG15200.047.2197889
GCGAAAG13900.045.9090422
GGTCGTT9300.035.8621837
GCTATTG32000.035.5710568
AGCTATC24950.032.2540979
GTAGAAA30700.031.8968871
CTATTGA36850.030.8883429
TAGAGCT39750.030.4645714
AGGTCGT12000.028.4009156
GAGCTAT41300.028.2677696
ACCGTGC28500.027.7286538
TATAACG1050.00770325727.6249262
TTAGAGC44950.027.2635563
CCCTTAT20000.025.7502381
AGAGCTA45350.025.2636175
GTCTAGT19950.024.3604221
ATCTATC9850.023.5537958
TATAGTA21600.022.828932
Produced by FastQC (version 0.11.5)