FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4344623
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCG1003432.309590498416088No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA621331.4301125782375133No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAA619891.4267981364551077No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT501931.1552901137797227No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT390720.8993185369593633No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGA317360.7304661417112601No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT310330.714285221065211No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC300430.691498433811173No Hit
GGATAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA281830.6486868941217684No Hit
GCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGAT255960.5891420268225805No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG240580.5537419472299437No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA235550.5421644179483467No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG233510.5374689587566056No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG218080.5019537943798575No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG216000.4971662673608274No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA206370.47500093794099046No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGA183820.423097700306793No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG177330.408159695329146No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA177150.4077453901063452No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA172520.39708853909763864No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG161210.371056360931662No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT158710.36530212172609683No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG142250.327416210796656No Hit
TGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGA141070.3247002098916293No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG137660.31685142761523843No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA136980.31528627455132474No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC135960.31293854495545415No Hit
ATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG132240.3043762370175732No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA131220.3020285074217026No Hit
ATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCT127970.294547996454468No Hit
CCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAG125710.2893461642126371No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT119680.275466939248814No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA118820.2734874809620996No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACA117390.2701960561365163No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT114040.26248537560105906No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG113580.2614265955872351No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG113510.2612654768894792No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT112970.2600225612210772No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC109650.2523809315560867No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA107450.24731720105518937No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT105890.24372655579091673No Hit
GCATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA94570.21767136066811782No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG94480.21746420805671746No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT93750.21578397020869244No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG90720.2088098322915475No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT89640.20632400095474338No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA88640.2040223052725173No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG88640.2040223052725173No Hit
CCATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAAT86300.19863633737610834No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT81380.18731199461955617No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC79070.181995077593614No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC78390.18042992452970028No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA78350.18033785670241123No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA77600.1786115849407417No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC77070.1773916862291619No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT76820.17681626230860537No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGC76550.17619480447440436No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG75040.172719243994243No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA73570.16933575134137072No Hit
CCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATTCGA71970.165653038249809No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC71650.16491649563149668No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC71550.16468632606327407No Hit
TCATATAGGAGACCGGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCG69480.15992181600106614No Hit
AAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG69210.1593003581668651No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA68750.15824157815304113No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT68040.15660737421866064No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG67580.15554859420483666No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA66990.15419059375232327No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG66710.1535461189613No Hit
GGCCTCCACCATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGG66320.15264845764523183No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG64870.14931099890600405No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT63600.14638784538957694No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC63100.14523699754846392No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA62990.14498381102341906No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG60490.13922957181785395No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC60370.13895336833598682No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT60130.13840096137225255No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT57930.13333723087135524No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT57690.13278482390762097No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT56030.12896400907512573No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG55820.12848065298185826No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA55600.12797427993176852No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG55100.1268234320906555No Hit
GGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGG54510.12546543163814214No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA54470.1253733638108531No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA54470.1253733638108531No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA54400.12521224511309728No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT54040.12438363466749588No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT52100.11991834504397736No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA51300.1180769884981965No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG51300.1180769884981965No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG50770.11685708978661669No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT50520.11628166586606019No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG50350.11589037760008176No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATT50080.11526891976588072No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC49930.11492366541354682No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG49170.113174376695055No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT48720.1121386136380533No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG48410.11142508797656321No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG48310.11119491840834062No Hit
GAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCC47840.11011312143769438No Hit
GCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTG47830.1100901044808721No Hit
GCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCG47480.10928451099209298No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC47320.10891623968293683No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG46780.10767332401453475No Hit
CAAACAGACTGTGTTCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGT46400.10679867965528884No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA45960.10578593355510939No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC45740.10527956050501966No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT44860.10325406830466073No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC44820.10316200047737169No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT43910.10106745740654596No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTAACCGGTCTCCTAT43600.1003539317450559No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA80250.0116.1370241
GCAGGTA12300.0103.7660142
CAGGTAC13100.097.42583
AGCGAAA8700.092.53721
GTAGAAA108750.085.8883362
TAGAAAC116350.080.71163
ACTGATC15550.080.664668
GGTACTG16000.078.859115
GCAGGCA26050.074.880234
GTACTGA18300.072.117876
CCGGAGA2250.070.90274
AGAAACA138600.067.75314
AGCAGGC29200.067.300613
GGTCAAA37050.065.5796661
GCGAAAG12150.065.057562
CGAAAGC12400.064.328533
ATACCGG2500.063.8256531
CGATCCG1250.063.812435
AGGTACT20600.063.7138674
GAGCGAA2200.062.6388131
Produced by FastQC (version 0.11.5)