FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5000881
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT87870.17570904006714017No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA84830.16963011117441107No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT77950.1558725352592873No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC66620.13321652724789892No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA65910.13179677740782073No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA64030.1280374398031067No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG62470.12491798945025885No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG61020.12201850034024005No Hit
CTTATAGACTTGTCTAATCACTGTATTATACATCTGAAACTAATGTAACA59280.11853911340821746No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT57910.1157995961111652No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC56640.11326004358032116No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC56100.11218023384279689No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA55260.11050052980664807No Hit
GTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCATACGTTTATTTTG54420.10882082577049923No Hit
ATATTCAACTGTCTCTCCTTTGACCGATGTTCGAGTTATGTTTTGTTTTC53360.1067011992486924No Hit
GCGTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTT53190.10636125914613845No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT52320.10462156568012716No Hit
ATGTATTAGTTTAGGGAAAATTAATGAAGATAAAAACAATAACAAGCACA51540.10306184050370325No Hit
GGTGTAGGGTTTGTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCAT51100.10218199553238719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9700.056.085071
GCGAAAG15050.041.449541
CGAAAGC16300.036.926632
GTAGAAA36500.034.7778661
GCTATTG29200.033.2714088
GCATTAA31600.032.5956041
CTATTGA31450.031.350919
GAGCTAT32900.029.9763776
GTCGTCG1255.157178E-428.999594145
GTCTAGT24050.027.1447071
CAATAGG50100.026.3506551
TAGAGCT43700.024.7252664
TAGAAAC57250.023.6872842
TAGTACT32600.023.3565044
ATAGGAG57650.023.2711163
GACAGTG31650.023.1376177
TTAGAGC47600.022.3952143
AGAGCTA45250.022.1154945
ACCGTGC36750.021.5039588
AATAGGA63150.021.3593862
Produced by FastQC (version 0.11.5)